SULT1A1 recombinant protein

SULT1A1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120901

• Gene Names: SULT1A1; PST; STP; STP1; P-PST; ST1A1; ST1A3; TSPST1; HAST1/HAST2
• Purity: >95% as determined by SDS-PAGE
• Synonyms: SULT1A1; SULT1A1 Protein; Human; Recombinant (His Tag); Human SULT1A1/STP1 Protein (His Tag); HAST1/HAST2 Protein; P-PST Protein; PST Protein; ST1A1 Protein; ST1A3 Protein; STP Protein; STP1 Protein; TSPST1 Protein; sulfotransferase family; cytosolic; 1A; phenol-preferring; member 1; SULT1A1 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 150mM NaCl, 10% glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Glu2-Leu295

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human SULT1A1 (P50225-1)(Glu 2-Leu 295) was fused with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for SULT1A1 recombinant protein

Background: Sulfate conjugation catalyzed by cytosolic sulfotransferase (SULT) enzymes. The SULTs are Phase II drug-metabolizing enzymes that catalyze the addition of a sulfuryl moiety to both endogenous compounds, including steroids and neurotransmitters, and certain xenobiotics, including N-hydroxy-2-acetylaminoflourine and phenolic compounds, like alpha-naphthol. SULTs may be involved in the individual genetic disposition, species differences, and organotropisms for toxicological effects of chemicals. Particularly SULT1A1 (Sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1), a member of the sulfotransferase 1 subfamily, which is a major pathway for drug metabolism in humans. Humans have at least 10 functional SULT genes. There has been an explosion in information on sulfotransferase polymorphisms and their functional consequences. An Arg213His polymorphism in SULT1A1 has a strong influence on the level of enzyme protein and activity in platelets, which have been widely used for phenotyping. Statistically significant associations were observed between the SULT1A1 genotype (Arg213His) and age, obesity and certain neoplasias (mammary, pulmonary, esophageal and urothelial cancer). Furthermore, the polymorphism of the SULT1A1 may be closely associated with breast cancer.

References

Coughtrie MW. 2002. Sulfation through the looking glass--recent advances in sulfotransferase research for the curious. Pharmacogenomics J. 2(5): 297-308.Klaassen CD, et al. (1998) Regulation of sulfotransferase mRNA expression in male and female rats of various ages. Chem Biol Interact. 109(1-3): 299-313.Glatt H. (2000) Sulfotransferases in the bioactivation of xenobiotics. Chem Biol Interact. 129(1-2): 141-70.Glatt H, et al. (2004) Pharmacogenetics of soluble sulfotransferases (SULTs). Naunyn Schmiedebergs Arch Pharmacol. 369(1): 55-68.Hebbring SJ, et al. (2008) Sulfotransferase gene copy number variation: pharmacogenetics and function. Cytogenet Genome Res. 123(1-4): 205-10.

NCBI and Uniprot Product Information

• NCBI GI #: 313104286
• NCBI GeneID: 6817
• UniProt Accession #: P50225; Q2NL71; Q86U58; Q92818; Q9BVU6; Q9UGG7
• Molecular Weight: Calculated MW: 25kDa/34kDa; Observe MW: 33kDa
• NCBI Official Full Name: Sulfotransferase 1A1
• NCBI Official Synonym Full Names: sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1
• NCBI Official Symbol: SULT1A1
• NCBI Official Synonym Symbols: PST; STP; STP1; P-PST; ST1A1; ST1A3; TSPST1; HAST1/HAST2
• NCBI Protein Information: sulfotransferase 1A1; ts-PST; P-PST 1; aryl sulfotransferase 1; thermostable phenol sulfotransferase1; phenol-sulfating phenol sulfotransferase 1
• UniProt Protein Name: Sulfotransferase 1A1
• Protein Family: Sulfotransferase
• UniProt Gene Name: SULT1A1
• UniProt Synonym Gene Names: STP; STP1; ST1A1; P-PST 1; Ts-PST
• UniProt Entry Name: ST1A1_HUMAN

NCBI Description

Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes one of two phenol sulfotransferases with thermostable enzyme activity. Multiple alternatively spliced variants that encode two isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SULT1A1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. Has also estrogen sulfotransferase activity. responsible for the sulfonation and activation of minoxidil. Is Mediates the metabolic activation of carcinogenic N- hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk. Belongs to the sulfotransferase 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.8.2.1; Energy Metabolism - sulfur; Transferase

Chromosomal Location of Human Ortholog: 16p12.1

Cellular Component: cytosol

Molecular Function: sulfotransferase activity; steroid sulfotransferase activity; flavonol 3-sulfotransferase activity; aryl sulfotransferase activity

Biological Process: estrogen metabolic process; flavonoid metabolic process; xenobiotic metabolic process; sulfation; catecholamine metabolic process; amine metabolic process; 3'-phosphoadenosine 5'-phosphosulfate metabolic process