Suppressor of Fused Homolog Recombinant Protein | SUFU recombinant protein

Recombinant Human Suppressor of Fused Homolog

Cat. Num. AAA144953

• Gene Names: SUFU; SUFUH; SUFUXL; PRO1280
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Suppressor of Fused Homolog; Recombinant Human Suppressor of Fused Homolog; SUFU Human; Suppressor of Fused Homolog Human Recombinant; Suppressor of fused homolog; SUFUH; SUFU; UNQ650/PRO1280; SUFUXL; PRO1280; SUFU recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: SUFU protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 10% glycerol and 50mM NaCl.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MAELRPSGAP GPTAPPAPGP TAPPAFASLF PPGLHAIYGE CRRLYPDQPN PLQVTAIVKY WLGGPDPLDY VSMYRNVGSP SANIPEHWHY ISFGLSDLYG DNRVHEFTGT DGPSGFGFEL TFRLKRETGE SAPPTWPAEL MQGLARYVFQ SENTFCSGDH VSWHSPLDNS ESRIQHMLLT EDPQMQPVQT PFGVVTFLQI VGVCTEELHS AQQWNGQGIL ELLRTVPIAG GPWLITDMRR GETIFEIDPH LQERVDKGIE TDGSNLSGVS AKCAWDDLSR PPEDDEDSRS ICIGTQPRRL SGKDTEQIRE TLRRGLEINS KPVLPPINPQ RQNGLAHDRA PSRKDSLESD SSTAIIPHEL IRTRQLESVH LKFNQESGAL IPLCLRGRLL HGRHFTYKSI TGDMAITFVS TGVEGAFATE EHPYAAHGPW LQILLTEEFV EKMLEDLEDL TSPEEFKLPK EYSWPEKKLK VSILPDVVFD SPLH
• Sequence Length: 433

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for SUFU recombinant protein

Description: SUFU Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 504 amino acids (1-484 a.a.) and having a molecular mass of 56.1kDa.SUFU is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: Suppressor of fused homolog (SUFU) is a member of the SUFU family. SUFU is a negative regulator of the hedgehog signaling pathway. SUFU interacts with GLI1, GLI3 and PEX26. Defects in the SUFU gene are a cause of medulloblastoma.

Product Categories/Family for SUFU recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 296011020
• NCBI GeneID: 51684
• NCBI Accession #: NP_001171604.1
• NCBI GenBank Nucleotide #: NM_001178133.1
• UniProt Accession #: Q9UMX1; Q7LCP7; Q9NT90; Q9NZ07; Q9UHK2; Q9UHM8; Q9UMY0
• Molecular Weight: 52,977 Da
• NCBI Official Full Name: suppressor of fused homolog isoform 2
• NCBI Official Synonym Full Names: suppressor of fused homolog (Drosophila)
• NCBI Official Symbol: SUFU
• NCBI Official Synonym Symbols: SUFUH; SUFUXL; PRO1280
• NCBI Protein Information: suppressor of fused homolog
• UniProt Protein Name: Suppressor of fused homolog
• Protein Family: Suppressor of fused
• UniProt Gene Name: SUFU
• UniProt Synonym Gene Names: SUFUH
• UniProt Entry Name: SUFU_HUMAN

NCBI Description

The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

SUFU: a transcriptional regulator which negatively regulates the hedgehog signaling pathway. Down-regulates GLI1-mediated transactivation of target genes. Part of a corepressor complex that acts on DNA-bound GLI1. Modulates nuclear-cytoplasmic shuttling of Gli-1. May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome. Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of FUSED to both SUFU and a GLI protein. Mutations in SUFU predispose to medulloblastoma. Part of a DNA-bound corepressor complex containing SAP18, GLI1 and SIN3. Part of a complex containing CTNNB1. Binds BTRC, GLI2, GLI3, SAP18 and STK36. Binds both free and DNA-bound GLI1. Defects in SUFU are a cause of medulloblastoma (MDB). MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children. Three alternatively spliced isoforms have been described.

Protein type: Adaptor/scaffold; Tumor suppressor; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 10q24.32

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: signal transducer activity; protein binding; beta-catenin binding; transcription factor binding; transcription corepressor activity; protein kinase binding

Biological Process: skin development; cytoplasmic sequestering of transcription factor; negative regulation of transcription factor activity; multicellular organismal development; negative regulation of transcription from RNA polymerase II promoter; proteolysis; negative regulation of transcription factor import into nucleus; signal transduction; regulation of transcription, DNA-dependent; negative regulation of smoothened signaling pathway; neural tube closure; negative regulation of osteoblast differentiation; heart looping; smoothened signaling pathway involved in ventral spinal cord interneuron specification; skeletal development; smoothened signaling pathway involved in spinal cord motor neuron cell fate specification

Disease: Meningioma, Familial, Susceptibility To; Basal Cell Nevus Syndrome; Medulloblastoma