Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SUFU antibody.)
Rabbit SUFU Polyclonal Antibody | anti-SUFU antibody
SUFU antibody
Immunofluorescence: 1:50 - 1:200
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SUFU antibody.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SUFU antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF7 cell using SUFU antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF7 cell using SUFU antibody. Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
NCBI Description
The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
Uniprot Description
SUFU: a transcriptional regulator which negatively regulates the hedgehog signaling pathway. Down-regulates GLI1-mediated transactivation of target genes. Part of a corepressor complex that acts on DNA-bound GLI1. Modulates nuclear-cytoplasmic shuttling of Gli-1. May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome. Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of FUSED to both SUFU and a GLI protein. Mutations in SUFU predispose to medulloblastoma. Part of a DNA-bound corepressor complex containing SAP18, GLI1 and SIN3. Part of a complex containing CTNNB1. Binds BTRC, GLI2, GLI3, SAP18 and STK36. Binds both free and DNA-bound GLI1. Defects in SUFU are a cause of medulloblastoma (MDB). MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children. Three alternatively spliced isoforms have been described.
Protein type: Tumor suppressor; Adaptor/scaffold; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 10q24.32
Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus
Molecular Function: protein binding; signal transducer activity; beta-catenin binding; protein kinase binding; transcription corepressor activity; transcription factor binding
Biological Process: skin development; cytoplasmic sequestering of transcription factor; negative regulation of transcription factor activity; multicellular organismal development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription factor import into nucleus; signal transduction; proteolysis; regulation of transcription, DNA-dependent; negative regulation of smoothened signaling pathway; neural tube closure; negative regulation of osteoblast differentiation; heart looping; smoothened signaling pathway involved in ventral spinal cord interneuron specification; skeletal development; smoothened signaling pathway involved in spinal cord motor neuron cell fate specification
Disease: Meningioma, Familial, Susceptibility To; Basal Cell Nevus Syndrome; Medulloblastoma
Research Articles on SUFU
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Product Notes
The SUFU sufu (Catalog #AAA9414663) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SUFU antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SUFU can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Western blotting: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200. Researchers should empirically determine the suitability of the SUFU sufu for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SUFU, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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