TMB substrate
TMB ELISA Substrate
Applications
ELISA
Synonyms
TMB; TMB ELISA Substrate; TMB substrate
Form/Format
Liquid ultra sensitive substate for ELISA mircowell applications containing TMB (3,3,5,5-tetramethylbenzidine) in a mildly acidic buffer. Mixed substrate/chromogen. Excellent for microwell peroxidase immunoassay systems
Concentration
Ready to use @ 100ul per well
Use this product at room temperature (varies by lot)
Use this product at room temperature (varies by lot)
Applicable Applications for TMB substrate
ELISA (EIA)
Application Notes
Optimal conditions to be determined by end user. This product should be allowed to equilibrate to room temperature.
Biological Significance
This HRP Microwell Substrate contains 3,3',5,5'-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a ready to use solution. Unreacted substrate should be colorless or very light yellow in appearance. When this substrate system is reacted with peroxidase, a soluble blue reaction product is obtained. The reaction can be stopped using appropriate stop solution, producing a soluble yellow or soluble blue reaction product, depending upon the stop reagent used, which is stable for at least 1 hour.
Biohazard
Non-hazardous, mild oxidizing agent, no organic solvents. Use laboratory practices when handling; in case of contact flush with water.
Note
In kinetic assays. TMB develops a deep blue color in the presence of peroxidase and can be read at 370 or 650nm. In endpoint assays, TMB develops a yellow color and can be read at 450nm approximately 30 to 60 minutes after adding stopping reagent.
Preparation and Storage
Store at 4 degree C. Product is light sensitive: protect from direct sunlight and UV sources.
Related Product Information for TMB substrate
High sensitivity HRP microwell substrate (containing TMB)
Product Categories/Family for TMB substrate
Similar Products
Product Notes
The TMB (Catalog #AAA539294) is a Substrate and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TMB can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Optimal conditions to be determined by end user. This product should be allowed to equilibrate to room temperature. Researchers should empirically determine the suitability of the TMB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TMB, Substrate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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