Rabbit anti-Human, Mouse KPNA4 Polyclonal Antibody | anti-KPNA4 antibody

KPNA4 Antibody

Cat. Num. AAA7048540

• Gene Names: KPNA4; QIP1; SRP3; IPOA3
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: KPNA4; Polyclonal Antibody; KPNA4 Antibody; Importin subunit alpha-3; Importin alpha Q1; Qip1; Karyopherin subunit alpha-4; QIP1; anti-KPNA4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 521

• Applicable Applications for anti-KPNA4 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1: 500-1: 5000; IHC: 1: 20-1: 200; IF: 1: 50-1: 200
• Immunogen: Recombinant Human Importin subunit alpha-3 protein (51-239AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugation: Non-conjugated
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-55266 / sc-55267 / sc-55270
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in:Mouse heart tissue,Mouse lung tissue,Mouse brain tissue,Mouse kidney tissue,Mouse skeletal muscle tissueAll lanes: KPNA4 antibody at 3.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 58 kDaObserved band size: 58 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human testis tissue using MBS7048540 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human prostate cancer using MBS7048540 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of PC3 cells using MBS7048540 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-KPNA4 antibody

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.

NCBI and Uniprot Product Information

• NCBI GI #: 4504901
• NCBI GeneID: 3840
• NCBI Accession #: NP_002259.1
• NCBI GenBank Nucleotide #: NM_002268.4
• UniProt Accession #: O00629; O00190; A8K4S6; D3DNM2
• Molecular Weight: 57,887 Da
• NCBI Official Full Name: importin subunit alpha-3
• NCBI Official Synonym Full Names: karyopherin subunit alpha 4
• NCBI Official Symbol: KPNA4
• NCBI Official Synonym Symbols: QIP1; SRP3; IPOA3
• NCBI Protein Information: importin subunit alpha-3
• UniProt Protein Name: Importin subunit alpha-3
• UniProt Gene Name: KPNA4
• UniProt Synonym Gene Names: QIP1; Qip1
• UniProt Entry Name: IMA3_HUMAN

NCBI Description

The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen. [provided by RefSeq, Jul 2008]

Uniprot Description

KPNA4: an alpha importin protein that functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Forms a complex with importin beta-1 subunit.

Protein type: Adaptor/scaffold; Chaperone; Karyopherin; Nuclear import

Chromosomal Location of Human Ortholog: 3q25.33

Cellular Component: cytosol; nuclear pore; nucleoplasm

Molecular Function: nuclear localization sequence binding; protein binding; protein transporter activity

Biological Process: modification by virus of host cellular process; NLS-bearing substrate import into nucleus