ECL substrate
ECL Western Blotting Substrate Kit
Applications
Western Blot
Synonyms
ECL; ECL Western Blotting Substrate Kit; ECL Western Blotting Substrate Set; ECL substrate
Form/Format
Luminol based for detection of horseraddish peroxidase. Mix detection reagents A&B at a 1:1 ratio. Immediately add the mixture to the blot. Incubate for 1min at room tempeture and drain excess reagents.
Concentration
25mL , ready to use reagents A&B (varies by lot)
Applicable Applications for ECL substrate
Western Blot (WB)
Biological Significance
ECL Western Blotting Substrate is a highly sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for easy detection of horseradish peroxidase (HRP) on immunoblots. ECL Western Blotting Substrate offers superior signal and clear background. Blots can be repeatedly exposed to X-ray film or a CCD camera to obtain optimal results or stripped of the immunodetection reagents and re-probed. The special formulation of ECL substrate makes it ideal substitute for Amersham ECL substrate without the need for additional optimization of assay conditions.
Biohazard
Use standard laboratory procedures when handling this product.
Preparation and Storage
Store at 4 degree C
Related Product Information for ECL substrate
ECL Western Blotting Substrate Kit for use in the research laboratory
Product Categories/Family for ECL substrate
Similar Products
Product Notes
The ECL (Catalog #AAA837823) is a Substrate and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ECL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the ECL for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ECL, Substrate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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