Rabbit anti-Human LKB1 Polyclonal Antibody | anti-LKB1 antibody

LKB1 (Phospho-Ser428) Antibody

Cat. Num. AAA9407749

• Gene Names: STK11; PJS; LKB1; hLKB1
• Reactivity: Human
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: LKB1; Polyclonal Antibody; LKB1 (Phospho-Ser428) Antibody; Protein kinase; CAMK group; CAMKL family; LKB subfamily; anti-LKB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of LKB1 only when phosphorylated at serine 428.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 433

• Applicable Applications for anti-LKB1 antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:1000
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of serine428 (R-L-S(p)-A-C) derived from Human LKB1.
• Modification: Phospho-Ser428
• Target Name: LKB1
• Preparation and Storage: Store at -20 degree C/1 year

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells treated with PMA using Phospho-LKB1 (Ser428) antibody.The lane on the right is treated with the antigen-specific peptide.)

Related Product Information for anti-LKB1 antibody

Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, leading to promote their activity: phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non-AMPK family proteins such as STRADA and possibly p53/TP53. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2: it thereby regulates inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neurons polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response: interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation. Able to phosphorylate p53/TP53; the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1 Also acts as a mediator p53/TP53-dependent apoptosis via interaction with p53/TP53: translocates to mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways.Zhu H, et al. (2013) J Biol Chem 288, 16495-505. Seto SW, et al. (2013) PLoS One 8, e66404. Rosario FJ, et al. (2012)J Physiol 590, 1495-509.

Product Categories/Family for anti-LKB1 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4507271
• NCBI GeneID: 6794
• NCBI Accession #: NP_000446.1
• NCBI GenBank Nucleotide #: NM_000455.4
• UniProt Accession #: Q15831; B2RBX7; E7EW76
• Molecular Weight: 45,387 Da
• NCBI Official Full Name: serine/threonine-protein kinase STK11
• NCBI Official Synonym Full Names: serine/threonine kinase 11
• NCBI Official Symbol: STK11
• NCBI Official Synonym Symbols: PJS; LKB1; hLKB1
• NCBI Protein Information: serine/threonine-protein kinase STK11
• UniProt Protein Name: Serine/threonine-protein kinase STK11
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: STK11
• UniProt Synonym Gene Names: LKB1; PJS; LKB1; hLKB1
• UniProt Entry Name: STK11_HUMAN

NCBI Description

This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

LKB1: a S/T protein kinase of the CAMKL family. A tumor suppressor that helps control cell structure, polarity, apoptosis and energy homeostasis. Phosphorylates AGS3 (activator of G-protein signaling 3) GPR domains, regulating the interaction of GPR-containing proteins with G-proteins. A cytosolic protein complex comprised of LKB1, the pseudokinase STRAD, and the MO25 scaffold protein. Activates AMPK and several related protein kinases. AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations. The Lkb1/Strad/Mo25 complex can polarize single epithelial cells, leading to the formation of a brush border containing microvilli on the apical surface. Mutation in the corresponding gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands and feet. A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues.

Protein type: Kinase, protein; Tumor suppressor; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; Autophagy; CAMK group; CAMKL family; LKB subfamily

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: membrane; mitochondrion; cytoplasm; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; p53 binding; protein complex binding; magnesium ion binding; protein kinase activator activity; LRR domain binding; ATP binding

Biological Process: Golgi localization; response to glucagon stimulus; protein heterooligomerization; protein amino acid autophosphorylation; Wnt receptor signaling pathway through beta-catenin; response to lipid; glucose homeostasis; protein amino acid phosphorylation; T cell receptor signaling pathway; anoikis; negative regulation of cell proliferation; positive regulation of gluconeogenesis; regulation of fatty acid biosynthetic process; vasculature development; tissue homeostasis; regulation of cell growth; cell cycle arrest; positive thymic T cell selection; positive regulation of transforming growth factor beta receptor signaling pathway; regulation of Wnt receptor signaling pathway; regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; axonogenesis; establishment of cell polarity; energy reserve metabolic process; insulin receptor signaling pathway; activation of protein kinase activity; autophagy; positive regulation of axonogenesis; response to ionizing radiation; negative regulation of cell growth; response to DNA damage stimulus; spermatid development

Disease: Pancreatic Cancer; Testicular Germ Cell Tumor; Peutz-jeghers Syndrome