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Immunohistochemistry (IHC) (Peptide - / +Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Stef in A antibody.)

anti-Human Stefin A Antibody | anti-STFA antibody

Stefin A Antibody

Gene Names
CSTA; AREI; PSS4; STF1; STFA
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
The antibody was Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Stefin A; Stefin A Antibody; STF1; STFA; cystatin A (stefin A); cystatin AS; anti-STFA antibody
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Stefin A antibody detects endogenous levels of total Stefin A protein.
Purity/Purification
The antibody was Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
98
Applicable Applications for anti-STFA antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:5000
Immunogen
The antiserum was produced against synthesized peptide derived from C-terminal of human Stefin A.
Preparation and Storage
Store at-20 degree C for 1 year.

Immunohistochemistry (IHC)

(Peptide - / +Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Stef in A antibody.)

Immunohistochemistry (IHC) (Peptide - / +Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Stef in A antibody.)

Western Blot (WB)

(Western blot analysis of extracts from A549 cells, using Stefin A antibody.)

Western Blot (WB) (Western blot analysis of extracts from A549 cells, using Stefin A antibody.)

Immunofluorescence (IF)

(Peptide - / +Immunofluorescence analysis of A549 cells, using Stefin A antibody.)

Immunofluorescence (IF) (Peptide - / +Immunofluorescence analysis of A549 cells, using Stefin A antibody.)
References
Janko Kos, Clin. Cancer Res., Feb 2000; 6: 505. Wendong Li, Clin. Cancer Res., Dec 2005; 11: 8753-8762. I Mlinaric-Rascan, Am. J. Pathol., Oct 1994; 145: 902.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cystatin-A
NCBI Official Synonym Full Names
cystatin A
NCBI Official Symbol
CSTA
NCBI Official Synonym Symbols
AREI; PSS4; STF1; STFA
NCBI Protein Information
cystatin-A
UniProt Protein Name
Cystatin-A
UniProt Gene Name
CSTA
UniProt Synonym Gene Names
STF1; STFA

NCBI Description

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

This is an intracellular thiol proteinase inhibitor. Has an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis.

Research Articles on STFA

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Product Notes

The STFA csta (Catalog #AAA852720) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Stefin An Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Stefin A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000. Researchers should empirically determine the suitability of the STFA csta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Stefin A, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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