anti-Human Stefin A Antibody | anti-STFA antibody

Stefin A Antibody

Cat. Num. AAA852720

• Gene Names: CSTA; AREI; PSS4; STF1; STFA
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Purity: The antibody was Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: Stefin A; Stefin A Antibody; STF1; STFA; cystatin A (stefin A); cystatin AS; anti-STFA antibody

• Reactivity: Human
• Specificity: Stefin A antibody detects endogenous levels of total Stefin A protein.
• Purity/Purification: The antibody was Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 98

• Applicable Applications for anti-STFA antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:5000
• Immunogen: The antiserum was produced against synthesized peptide derived from C-terminal of human Stefin A.
• Preparation and Storage: Store at-20 degree C for 1 year.

Immunohistochemistry (IHC)

(Peptide - / +Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Stef in A antibody.)

Western Blot (WB)

(Western blot analysis of extracts from A549 cells, using Stefin A antibody.)

Immunofluorescence (IF)

(Peptide - / +Immunofluorescence analysis of A549 cells, using Stefin A antibody.)

Product Categories/Family for anti-STFA antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Janko Kos, Clin. Cancer Res., Feb 2000; 6: 505. Wendong Li, Clin. Cancer Res., Dec 2005; 11: 8753-8762. I Mlinaric-Rascan, Am. J. Pathol., Oct 1994; 145: 902.

NCBI and Uniprot Product Information

• NCBI GI #: 4885165
• NCBI GeneID: 1475
• NCBI Accession #: NP_005204.1
• NCBI GenBank Nucleotide #: NM_005213.3
• UniProt Accession #: P01040; Q6IB90
• NCBI Official Full Name: cystatin-A
• NCBI Official Synonym Full Names: cystatin A
• NCBI Official Symbol: CSTA
• NCBI Official Synonym Symbols: AREI; PSS4; STF1; STFA
• NCBI Protein Information: cystatin-A
• UniProt Protein Name: Cystatin-A
• UniProt Gene Name: CSTA
• UniProt Synonym Gene Names: STF1; STFA

NCBI Description

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

This is an intracellular thiol proteinase inhibitor. Has an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis.