Human Stanniocalcin-1 ELISA Kit | STC1 elisa kit

Human Stanniocalcin-1 ELISA Kit

Cat. Num. AAA2888247

• Gene Names: STC1; STC
• Reactivity: Human
• Synonyms: Stanniocalcin-1; Human Stanniocalcin-1 ELISA Kit; STC1 elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human ENPP1
• Sequence Length: 247

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids
• Detection Range: 0.156 ng/ml-10 ng/mL
• Sensitivity: <0.04 ng/ml
• Intra-assay Precision: Intra-Assay CV: <=7.9%
• Inter-assay Precision: Inter-Assay CV: <=11%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for STC1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4507265
• NCBI GeneID: 6781
• NCBI Accession #: NP_003146.1
• NCBI GenBank Nucleotide #: NM_003155.2
• UniProt Accession #: P52823; Q71UE5; B4DN22
• Molecular Weight: 20,487 Da
• NCBI Official Full Name: stanniocalcin-1
• NCBI Official Synonym Full Names: stanniocalcin 1
• NCBI Official Symbol: STC1
• NCBI Official Synonym Symbols: STC
• NCBI Protein Information: stanniocalcin-1
• UniProt Protein Name: Stanniocalcin-1
• Protein Family: Stanniocalcin
• UniProt Gene Name: STC1
• UniProt Synonym Gene Names: STC; STC-1

NCBI Description

This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The gene contains a 5' UTR rich in CAG trinucleotide repeats. The encoded protein contains 11 conserved cysteine residues and is phosphorylated by protein kinase C exclusively on its serine residues. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Overexpression of human stanniocalcin 1 in mice produces high serum phosphate levels, dwarfism, and increased metabolic rate. This gene has altered expression in hepatocellular, ovarian, and breast cancers. [provided by RefSeq, Jul 2008]

Uniprot Description

STC1: Stimulates renal phosphate reabsorption, and could therefore prevent hypercalcemia. Belongs to the stanniocalcin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p21.2

Cellular Component: apical plasma membrane; cytoplasm; extracellular space; nucleus

Molecular Function: hormone activity

Biological Process: cellular calcium ion homeostasis; decidualization; embryo implantation; endothelial cell morphogenesis; negative regulation of calcium ion transport; negative regulation of cell migration; ossification; response to vitamin D