Human Stanniocalcin 1/STC1 PicoKine ELISA Kit | STC1 elisa kit

Human Stanniocalcin 1/STC1 PicoKine ELISA Kit

Cat. Num. AAA177578

• Gene Names: STC1; STC
• Reactivity: Human
• Synonyms: Stanniocalcin 1/STC1 PicoKine; Human Stanniocalcin 1/STC1 PicoKine ELISA Kit; Stanniocalcin-1; Stanniocalcin; STC; STC-1; STC1; STC1_HUMAN ; stanniocalcin 1; STC1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human STC1
• Sequence Length: 247

• Assay Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of human STC1 in cell culture supernates, serum and plasma(heparin, EDTA).
• Immunogen: NSO, V34-A247
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human STC1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum and plasma (heparin, EDTA) to each empty well. It is recommended that each human STC1 standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|246|801|1351|254|775|1416; Standard deviation|14.02|48.06|74.30|15.74|49.6|67.96; CV(%)|5.7|6.0|5.5|6.2|6.4|6.8
• Sample Data: Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000; O.D.|0.054|0.094|0.162|0.282|0.470|0.812|1.285|1.777
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Human Stanniocalcin 1/STC1 PicoKine ELISA Kit standard curve )

Related Product Information for STC1 elisa kit

Principle of the assay: Human STC1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for STC1 has been precoated onto 96-well plates. Standards (NSO, V34-A247) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for STC1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human STC1 amount of sample captured in plate.

Background: Stanniocalcin-1 is a glycoprotein which is encoded by the STC1 gene. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. Human Stanniocalcin-1 is a putative molecular biomarker of leukemic microenvironment and the only molecular function known up to date is a SUMO E3 ligase activity in the SUMOylation cycle. STC1 interacts with lots of proteins in the cytoplasm, mitochondria, endoplasmatic reticulum and dot-like fashion in the nucleus. The N-terminal region of STC1 is the function region which is responsible to establish the interaction with its partners, including SUMO1. Low resolution studies shows that STC1 is an anti-parallel homodimer in solution and the cystein 202 is responsible for the dimerization of this protein. All the 5 dissulfide bonds of human STC1 are conserved and have the same profile of fish STC.

NCBI and Uniprot Product Information

• NCBI GI #: 4507265
• NCBI GeneID: 6781
• NCBI Accession #: NP_003146.1
• NCBI GenBank Nucleotide #: NM_003155.2
• UniProt Accession #: P52823; Q71UE5; B4DN22
• Molecular Weight: 20,487 Da
• NCBI Official Full Name: stanniocalcin-1
• NCBI Official Synonym Full Names: stanniocalcin 1
• NCBI Official Symbol: STC1
• NCBI Official Synonym Symbols: STC
• NCBI Protein Information: stanniocalcin-1
• UniProt Protein Name: Stanniocalcin-1
• Protein Family: Stanniocalcin
• UniProt Gene Name: STC1
• UniProt Synonym Gene Names: STC; STC-1
• UniProt Entry Name: STC1_HUMAN

NCBI Description

This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The gene contains a 5' UTR rich in CAG trinucleotide repeats. The encoded protein contains 11 conserved cysteine residues and is phosphorylated by protein kinase C exclusively on its serine residues. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Overexpression of human stanniocalcin 1 in mice produces high serum phosphate levels, dwarfism, and increased metabolic rate. This gene has altered expression in hepatocellular, ovarian, and breast cancers. [provided by RefSeq, Jul 2008]

Uniprot Description

STC1: Stimulates renal phosphate reabsorption, and could therefore prevent hypercalcemia. Belongs to the stanniocalcin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8p21.2

Cellular Component: apical plasma membrane; cytoplasm; extracellular space; nucleus

Molecular Function: hormone activity

Biological Process: cellular calcium ion homeostasis; decidualization; embryo implantation; endothelial cell morphogenesis; negative regulation of calcium ion transport; negative regulation of cell migration; ossification; response to vitamin D