STAT3 recombinant protein

Recombinant STAT3

Cat. Num. AAA201088

• Gene Names: STAT3; APRF; HIES; ADMIO; ADMIO1
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: STAT3; Recombinant STAT3; Signal transducer and activator of transcription 3 isoform 1; ADMIO; ADMIO1; APRF; HIES; STAT3 recombinant protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid; In Phosphate Buffered Saline (pH 7.4) Containing 30% Glycerol, 1mM DTT.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 1-770aa
• Sequence: MAQWNQLQQL DTRYLEQLHQ LYSDSFPMEL RQFLAPWIES QDWAYAASKE SHATLVFHNL LGEIDQQYSR FLQESNVLYQ HNLRRIKQFL QSRYLEKPME IARIVARCLW EESRLLQTAA TAAQQGGQAN HPTAAVVTEK QQMLEQHLQD VRKRVQDLEQ KMKVVENLQD DFDFNYKTLK SQGDMQDLNG NNQSVTRQKM QQLEQMLTAL DQMRRSIVSE LAGLLSAMEY VQKTLTDEEL ADWKRRQQIA CIGGPPNICL DRLENWITSL AESQLQTRQQ IKKLEELQQK VSYKGDPIVQ HRPMLEERIV ELFRNLMKSA FVVERQPCMP MHPDRPLVIK TGVQFTTKVR LLVKFPELNY QLKIKVCIDK DSGDVAALRG SRKFNILGTN TKVMNMEESN NGSLSAEFKH LTLREQRCGN GGRANCDASL IVTEELHLIT FETEVYHQGL KIDLETHSLP VVVISNICQM PNAWASILWY NMLTNNPKNV NFFTKPPIGT WDQVAEVLSW QFSSTTKRGL SIEQLTTLAE KLLGPGVNYS GCQITWAKFC KENMAGKGFS FWVWLDNIID LVKKYILALW NEGYIMGFIS KERERAILST KPPGTFLLRF SESSKEGGVT FTWVEKDISG KTQIQSVEPY TKQQLNNMSF AEIIMGYKIM DATNILVSPL VYLYPDIPKE EAFGKYCRPE SQEHPEADPG SAAPYLKTKF ICVTPTTCSN TIDLPMSPRT LDSLMQFGNN GEGAEPSAGG QFESLTFDME LTSECATSPM HHHHHH
• Sequence Length: 769

• Applicable Applications for STAT3 recombinant protein: SDS-PAGE
• Species: Human
• Endotoxin: <1.0 EU per 1ug of protein (determined by LAL method)
• Tag: His-Tag
• Predicted N-Terminal: Met 1
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )

Related Product Information for STAT3 recombinant protein

STAT3, also known as signal transducer and activator of transcription 3 isoform 1, is a member of the STAT protein family. It is a transcription factor activated in response to cytokine, growth factor, or hormone receptor signaling. Once this protein is activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene and it mediates cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. It also acts as a regulator of inflammatory response by regulating differentiation of naive CD4+ T-cells into T-helper Th17 or regulatory T-cells. Recombinant human STAT3 protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for STAT3 recombinant protein

Signal Transduction

References

Chakraborty D., et al, (2017) Nat Commun. 8:1130. Han S., et al, (2018) Am J Cancer Res. 8:489-501.

NCBI and Uniprot Product Information

• NCBI GI #: 21618338
• NCBI GeneID: 6774
• NCBI Accession #: NP_003141.2
• NCBI GenBank Nucleotide #: NP_003141.2
• UniProt Accession #: P40763
• Molecular Weight: 88.8kDa (776aa); 70-100KDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: signal transducer and activator of transcription 3 isoform 2
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 3
• NCBI Official Symbol: STAT3
• NCBI Official Synonym Symbols: APRF; HIES; ADMIO; ADMIO1
• NCBI Protein Information: signal transducer and activator of transcription 3
• UniProt Protein Name: Signal transducer and activator of transcription 3
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT3
• UniProt Synonym Gene Names: APRF
• UniProt Entry Name: STAT3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

STAT3: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Constitutively active in a number of human tumors. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: Transcription factor; Nuclear receptor co-regulator; Motility/polarity/chemotaxis; DNA-binding

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: nucleoplasm; cytoplasm; mitochondrial inner membrane; plasma membrane; cytosol; nucleus

Molecular Function: protein dimerization activity; protein binding; ligand-dependent nuclear receptor activity; signal transducer activity; DNA binding; sequence-specific DNA binding; protein kinase binding; transcription factor binding; protein phosphatase binding; transcription factor activity; CCR5 chemokine receptor binding; glucocorticoid receptor binding

Biological Process: transcription from RNA polymerase II promoter; nerve growth factor receptor signaling pathway; viral reproduction; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; radial glial cell differentiation; thermoregulation; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; signal transduction; response to estradiol stimulus; negative regulation of cell proliferation; astrocyte differentiation; regulation of transcription, DNA-dependent; protein import into nucleus; acute-phase response; negative regulation of glycolysis; positive regulation of Notch signaling pathway; response to drug; nervous system development; intracellular receptor-mediated signaling pathway; eating behavior; cytokine and chemokine mediated signaling pathway; regulation of multicellular organism growth; JAK-STAT cascade; cellular response to hormone stimulus; regulation of transcription from RNA polymerase II promoter; cell proliferation; response to ethanol; sexual reproduction; positive regulation of transcription from RNA polymerase II promoter; eye photoreceptor cell differentiation; cell motility; phosphorylation

Disease: Hyper-ige Recurrent Infection Syndrome, Autosomal Dominant; Autoimmune Disease, Multisystem, Infantile-onset