Senescence beta-Galactosidase Staining Kit
Senescence beta-Galactosidase Staining Kit
Synonyms
Senescence beta-Galactosidase; Senescence beta-Galactosidase Staining Kit ; Cell Senescence; beta-Galactosidase Staining Kit ; Senescence beta-Galactosidase staining kit
Application Notes
Senescence beta-Galactosidase Staining Kit provide reagents for detecting the senescent cells or tissues based on the up-regulation of senescence-associated beta-galactosidase (SA-beta-Gal) activity at pH 6 during aging. The aging of cells or tissues can be observed under a common optical microscope. SA-beta-Gal is present only in senescent cells and is undetectable in presenescent, quiescent, tumor or immortal cells.
Preparation and Storage
Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment.
Shipping: Gel pack with blue ice.
Shipping: Gel pack with blue ice.
Related Product Information for Senescence beta-Galactosidase staining kit
Principle of the Assay: Senescence beta-Galactosidase Staining Kit is a kit for staining and detecting senescent cells or tissues based on the up-regulation of senescence-related beta-galactosidase activity level during aging. With X-Gal as the substrate, under the condition of pH 6.0, the senescent beta-galactosidase catalyzes the formation of dark blue products. So that cells or tissues expressing beta-galactosidase that turned blue were observed under an optical microscope. This kit is suitable for the detection of senescence in cultured cells and tissue sections. It only stains senescent cells, and does not stain pre-senescence cells (senescent cells), quiescent cells (static cells), immortal cells (immortal cells) or tumor cells.
Background/Introduction: Cells are the basic unit of biological structure and function, as well as the basic unit of biological aging. Cellular senescence is morphologically manifested as degeneration of cell structure, such as nuclear membrane depression, which eventually leads to nuclear membrane collapse, chromatin structure changes, and the number of hyperdiploid and abnormal polyploid cells increases; cell membrane fragility increases. Sexual permeability decreases, and the types and numbers of membrane receptors and the sensitivity to ligands change; lipofuscin accumulates in cells, and many organelles and intracellular structures undergo degeneration. Cell senescence is physiologically manifested as functional decline and low metabolism, such as cell cycle arrest, loss of cell replication ability, weakened responsiveness to mitogenic stimulation, and changes in responsiveness to pro-apoptotic factors; intracellular enzyme active centers are oxidized, Enzyme activity decreased, protein synthesis decreased, etc. The senescent cells can no longer replicate, but they still maintain metabolically active and stain positive for senescence-associated beta-galactosidase activity, which is considered to be a biomarker of cellular senescence.
Background/Introduction: Cells are the basic unit of biological structure and function, as well as the basic unit of biological aging. Cellular senescence is morphologically manifested as degeneration of cell structure, such as nuclear membrane depression, which eventually leads to nuclear membrane collapse, chromatin structure changes, and the number of hyperdiploid and abnormal polyploid cells increases; cell membrane fragility increases. Sexual permeability decreases, and the types and numbers of membrane receptors and the sensitivity to ligands change; lipofuscin accumulates in cells, and many organelles and intracellular structures undergo degeneration. Cell senescence is physiologically manifested as functional decline and low metabolism, such as cell cycle arrest, loss of cell replication ability, weakened responsiveness to mitogenic stimulation, and changes in responsiveness to pro-apoptotic factors; intracellular enzyme active centers are oxidized, Enzyme activity decreased, protein synthesis decreased, etc. The senescent cells can no longer replicate, but they still maintain metabolically active and stain positive for senescence-associated beta-galactosidase activity, which is considered to be a biomarker of cellular senescence.
Product Categories/Family for Senescence beta-Galactosidase staining kit
Similar Products
Product Notes
The Senescence beta-Galactosidase (Catalog #AAA9719021) is a Staining Kit and is intended for research purposes only. The product is available for immediate purchase. Senescence beta-Galactosidase Staining Kit provide reagents for detecting the senescent cells or tissues based on the up-regulation of senescence-associated beta-galactosidase (SA-beta-Gal) activity at pH 6 during aging. The aging of cells or tissues can be observed under a common optical microscope. SA-beta-Gal is present only in senescent cells and is undetectable in presenescent, quiescent, tumor or immortal cells. Researchers should empirically determine the suitability of the Senescence beta-Galactosidase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Senescence beta-Galactosidase, Staining Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
