Human Somatostatin Receptor 1 ELISA Kit | SSTR1 elisa kit

Human Somatostatin Receptor 1 ELISA Kit

Cat. Num. AAA27537

• Gene Names: SSTR1; SS1R; SS1-R; SRIF-2; SS-1-R
• Reactivity: Human
• Synonyms: Somatostatin Receptor 1; Human Somatostatin Receptor 1 ELISA Kit; SSTR1 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human SSTR1 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 6.25pg/ml-200pg/ml.
• Sensitivity: 1.0pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated SSTR1 concentrations in Human serum, plasma and other body fluids. Using Purified Human SSTR1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SSTR1 and SSTR1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SSTR1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for SSTR1 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated SSTR1 concentrations in Human serum, plasma and other body fluids. Using Purified Human SSTR1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SSTR1 and SSTR1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SSTR1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557857
• NCBI GeneID: 6751
• NCBI Accession #: NP_001040.1
• NCBI GenBank Nucleotide #: NM_001049.2
• UniProt Accession #: P30872
• Molecular Weight: 42,686 Da
• NCBI Official Full Name: somatostatin receptor type 1
• NCBI Official Synonym Full Names: somatostatin receptor 1
• NCBI Official Symbol: SSTR1
• NCBI Official Synonym Symbols: SS1R; SS1-R; SRIF-2; SS-1-R
• NCBI Protein Information: somatostatin receptor type 1
• UniProt Protein Name: Somatostatin receptor type 1
• Protein Family: Somatostatin receptor
• UniProt Gene Name: SSTR1
• UniProt Synonym Gene Names: SS-1-R; SS1-R; SS1R
• UniProt Entry Name: SSR1_HUMAN

NCBI Description

Somatostatins are peptide hormones that regulate diverse cellular functions such as neurotransmission, cell proliferation, and endocrine signaling as well as inhibiting the release of many hormones and other secretory proteins. Somatostatin has two active forms of 14 and 28 amino acids. The biological effects of somatostatins are mediated by a family of G-protein coupled somatostatin receptors that are expressed in a tissue-specific manner. The protein encoded by this gene is a member of the superfamily of somatostatin receptors having seven transmembrane segments. Somatostatin receptors form homodimers and heterodimers with other members of the superfamily as well as with other G-protein coupled receptors and receptor tyrosine kinases. This somatostatin receptor has greater affinity for somatostatin-14 than -28. [provided by RefSeq, Jul 2012]

Uniprot Description

SSTR1: Receptor for somatostatin with higher affinity for somatostatin-14 than -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and Na(+)/H(+) exchanger via pertussis toxin insensitive G proteins. Belongs to the G-protein coupled receptor 1 family. Interacts with SKB1.

Protein type: Cell cycle regulation; Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 14q13

Cellular Component: neuron projection; integral to plasma membrane; cytoplasm; plasma membrane

Molecular Function: neuropeptide binding; somatostatin receptor activity

Biological Process: glutamate signaling pathway; response to starvation; synaptic transmission; G-protein signaling, coupled to cyclic nucleotide second messenger; negative regulation of cell proliferation; cell surface receptor linked signal transduction; cell-cell signaling; neuropeptide signaling pathway; forebrain development; digestion; cerebellum development; spermatogenesis; response to nutrient