Probable DNA repair helicase RAD25 homolog (RAD25) Recombinant Protein | RAD25 recombinant protein

Recombinant Encephalitozoon cuniculi Probable DNA repair helicase RAD25 homolog (RAD25), partial

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Probable DNA repair helicase RAD25 homolog (RAD25); Recombinant Encephalitozoon cuniculi Probable DNA repair helicase RAD25 homolog (RAD25); partial; RAD25 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 672

• Species: Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 85691085
• NCBI GeneID: 860283
• NCBI Accession #: XP_965942.1
• NCBI GenBank Nucleotide #: XM_960849.1
• UniProt Accession #: Q8SSK1; CAD24977
• Molecular Weight: 76,626 Da
• NCBI Official Full Name: DNA repair helicase
• NCBI Official Symbol: rad25
• NCBI Protein Information: DNA repair helicase
• UniProt Protein Name: General transcription and DNA repair factor IIH helicase subunit XPB
• Protein Family: Putative DNA helicase
• UniProt Gene Name: SSL2
• UniProt Synonym Gene Names: RAD25; TFIIH subunit XPB; TFB subunit SSL2

Uniprot Description

ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/SSL2, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/SSL2 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.