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Western Blot (WB)
(Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 7.5% SDS PAGE gel and STK4 antibody at a dilution of 1:2000)
Rabbit STK4 Polyclonal Antibody | anti-STK4 antibody
STK4 Antibody
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
STK4; Polyclonal Antibody; STK4 Antibody; Affinity Purified Rabbit Polyclonal STK4 Antibody; Polyclonal STK4 antibody; Anti-STK4 antibody; DKFZp686A2068 antibody; mammalian sterile 20-like 1 antibody; STK4 antibody; STK-4; STK-4 antibody; serine/threonine-protein kinase Krs-2 antibody; YSK3 antibody; MST1 antibody; kinase responsive to stress 2 antibody; STK 4 antibody; MST-1 antibody; KRS2 antibody; STK 4; anti-STK4 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
462
Applicable Applications for anti-STK4 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IF: 1:100-1:1000
ICC: 1:100-1:1000
IHC-P: 1:100-1:1000
WB: 1:500-1:3000
ICC: 1:100-1:1000
IHC-P: 1:100-1:1000
WB: 1:500-1:3000
Cross-Reactivity
Human, Mouse
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 7.5% SDS PAGE gel and STK4 antibody at a dilution of 1:2000)
Western Blot (WB)
(Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 7.5% SDS PAGE gel and STK4 antibody at a dilution of 1:2000)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded Cal27 xenograft using STK4 antibody at a dilution of 1:500)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded Cal27 xenograft using STK4 antibody at a dilution of 1:500)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hep G2) using a 7.5% SDS PAGE gel and STK4 antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hep G2) using a 7.5% SDS PAGE gel and STK4 antibody at a dilution of 1:1000)
Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using STK4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.)
Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using STK4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.)
Related Product Information for anti-STK4 antibody
The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process.
Product Categories/Family for anti-STK4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56 kDa
NCBI Official Full Name
serine/threonine-protein kinase 4 isoform 2
Protein Family
Similar Products
Product Notes
The STK4 (Catalog #AAA5312250) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's STK4 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF: 1:100-1:1000 ICC: 1:100-1:1000 IHC-P: 1:100-1:1000 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the STK4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STK4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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