Typical Testing Data/Standard Curve
Mouse Sushi Repeat Containing Protein X Linked 2 ELISA Kit | SRPX2 elisa kit
Mouse Sushi Repeat Containing Protein X Linked 2 ELISA Kit
Gene Names
SRPX; DRS; ETX1; SRPX1; HEL-S-83p
Reactivity
Mouse
Synonyms
Sushi Repeat Containing Protein X Linked 2; Mouse Sushi Repeat Containing Protein X Linked 2 ELISA Kit; SRPX2 elisa kit
Reactivity
Mouse
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Detection Range
250-5000pg/mL
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Related Product Information for SRPX2 elisa kit
Intended Uses: This SRPX2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SRPX2. This ELISA kit for research use only!
Principle of the Assay||SRPX2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SRPX2 antibody and an SRPX2-HRP conjugate. The assay sample and buffer are incubated together with SRPX2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SRPX2 concentration since SRPX2 from samples and SRPX2-HRP conjugate compete for the anti-SRPX2 antibody binding site. Since the number of sites is limited, as more sites are occupied by SRPX2 from the sample, fewer sites are left to bind SRPX2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SRPX2 concentration in each sample is interpolated from this standard curve.
Principle of the Assay||SRPX2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SRPX2 antibody and an SRPX2-HRP conjugate. The assay sample and buffer are incubated together with SRPX2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SRPX2 concentration since SRPX2 from samples and SRPX2-HRP conjugate compete for the anti-SRPX2 antibody binding site. Since the number of sites is limited, as more sites are occupied by SRPX2 from the sample, fewer sites are left to bind SRPX2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SRPX2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for SRPX2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49,382 Da
NCBI Official Full Name
sushi repeat-containing protein SRPX isoform 2
NCBI Official Synonym Full Names
sushi-repeat containing protein, X-linked
NCBI Official Symbol
SRPX
NCBI Official Synonym Symbols
DRS; ETX1; SRPX1; HEL-S-83p
NCBI Protein Information
sushi repeat-containing protein SRPX; sushi-repeat-containing protein, X-linked; sushi-repeat-containing protein, X chromosome; epididymis secretory sperm binding protein Li 83p
UniProt Protein Name
Sushi repeat-containing protein SRPX
Protein Family
UniProt Gene Name
SRPX
UniProt Synonym Gene Names
ETX1
UniProt Entry Name
SRPX_HUMAN
Uniprot Description
SRPX: May be involved in phagocytosis during disk shedding, cell adhesion to cells other than the pigment epithelium or signal transduction. 3 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: Xp21.1
Cellular Component: cell surface; membrane; endoplasmic reticulum; autophagic vacuole
Biological Process: phagolysosome formation; autophagy; cell adhesion
Research Articles on SRPX2
Similar Products
Product Notes
The Mouse SRPX2 srpx (Catalog #AAA732781) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA732781 ELISA Kit recognizes Mouse SRPX2. It is sometimes possible for the material contained within the vial of "Sushi Repeat Containing Protein X Linked 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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