SDS-Page
SPSB2 recombinant protein
SPSB2, 1-263aa, Human, His tag, E Coli
Gene Names
SPSB2; SSB2; GRCC9
Applications
SDS-Page
Purity
> 85% by SDS-PAGE
Synonyms
SPSB2; 1-263aa; Human; His tag; E Coli; SPRY domain-containing SOCS box protein 2; GRCC9; SSB2; SPSB2 recombinant protein
Host
E Coli
Purity/Purification
> 85% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
Concentration
0.25 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMGQTAL AGGSSSTPTP QALYPDLSCP EGLEELLSAP PPDLGAQRRH GWNPKDCSEN IEVKEGGLYF ERRPVAQSTD GARGKRGYSR GLHAWEISWP LEQRGTHAVV GVATALAPLQ TDHYAALLGS NSESWGWDIG RGKLYHQSKG PGAPQYPAGT QGEQLEVPER LLVVLDMEEG TLGYAIGGTY LGPAFRGLKG RTLYPAVSAV WGQCQVRIRY LGERRAEPHS LLHLSRLCVR HNLGDTRLGQ VSALPLPPAM KRYLLYQ
Sequence Length
263
Applicable Applications for SPSB2 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for SPSB2 recombinant protein
SPSB2 is a suppressor of cytokine signaling (SOCS) family member, and it belongs to the subfamily of proteins containing a central SPRY (repeats in splA and RyR) domain and a C-terminal SOCS box. This protein is present in a gene-rich cluster on chromosome 12p13 in the vicinity of the CD4 antigen and triosephosphate isomerase genes. Recombinant human SPSB2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for SPSB2 recombinant protein
References
Wang,D., et al. (2005) Sect J. Biol. Chem. 280 (16), 16393-16401
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31.2 kDa (287aa), confirmed by MALDI-TOF
NCBI Official Full Name
SPRY domain-containing SOCS box protein 2
NCBI Official Synonym Full Names
splA/ryanodine receptor domain and SOCS box containing 2
NCBI Official Symbol
SPSB2
NCBI Official Synonym Symbols
SSB2; GRCC9
NCBI Protein Information
SPRY domain-containing SOCS box protein 2
UniProt Protein Name
SPRY domain-containing SOCS box protein 2
Protein Family
UniProt Gene Name
SPSB2
UniProt Synonym Gene Names
GRCC9; SSB2; SSB-2
NCBI Description
This gene encodes a member of a subfamily of proteins containing a central SPRY (repeats in splA and RyR) domain and a C-terminal suppressor of cytokine signaling (SOCS) box. This protein plays a role in cell signaling. This gene is present in a gene-rich cluster on chromosome 12p13 in the vicinity of the CD4 antigen and triosephosphate isomerase genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
Uniprot Description
Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Research Articles on SPSB2
1. crystal structure of SPSB2 in complex with cyclic peptide inhibitor cR8, at a resolution of 1.34 A
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Product Notes
The SPSB2 spsb2 (Catalog #AAA204684) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's SPSB2 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the SPSB2 spsb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSHMGQTAL AGGSSSTPTP QALYPDLSCP EGLEELLSAP PPDLGAQRRH GWNPKDCSEN IEVKEGGLYF ERRPVAQSTD GARGKRGYSR GLHAWEISWP LEQRGTHAVV GVATALAPLQ TDHYAALLGS NSESWGWDIG RGKLYHQSKG PGAPQYPAGT QGEQLEVPER LLVVLDMEEG TLGYAIGGTY LGPAFRGLKG RTLYPAVSAV WGQCQVRIRY LGERRAEPHS LLHLSRLCVR HNLGDTRLGQ VSALPLPPAM KRYLLYQ. It is sometimes possible for the material contained within the vial of "SPSB2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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