SPP1 (Osteopontin) Recombinant Protein | SPP1 recombinant protein

Human SPP1 (Osteopontin) Recombinant Protein with GST

Cat. Num. AAA698040

• Gene Names: SPP1; OPN; BNSP; BSPI; ETA-1
• Applications: ELISA, Western Blot
• Purity: >90%; Purification: GST tag, Glutathione Sepharose affinity chromatography
• Synonyms: SPP1 (Osteopontin); Human SPP1 (Osteopontin) Recombinant Protein with GST; Osteopontin; SPP1 protein; Bone sialoprotein-1; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Nephropontin; Uropontin; Secreted phosphoprotein 1 isoform b; SPP1; OPN; Early T lymphocyte activation 1; ETA1; SPP1 recombinant protein

• Host: E Coli
• Purity/Purification: >90%; Purification: GST tag, Glutathione Sepharose affinity chromatography
• Form/Format: Lyophilized; Phosphate-Buffered Saline (PBS), pH 7.4. No preservatives added
• Sequence Positions: 59-300 aa (Partial length)
• Sequence Length: 487; Length with TAG (aa)

• Applicable Applications for SPP1 recombinant protein: ELISA (EIA), Western Blot (WB), Antibody Production, Protein Array
• Organism Species: Human
• Biological Activity: Not tested
• Endotoxin Level: Not tested
• Quality Control: 10% SDS-PAGE stained with Coomassie Blue (CB) immunoblotting with anti-GST (WB) and peptide fingerprinting by MALDI-TOF mass spectrometry
• Preparation and Storage: Prior to reconstitution, store the lyophilized protein at 2-8 degree C. After reconstitution, store in working aliquots at -80 degree C.

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Product Categories/Family for SPP1 recombinant protein

Cytokine

NCBI and Uniprot Product Information

• NCBI GI #: 4759166
• NCBI GeneID: 6696
• NCBI Accession #: NP_000573.1
• NCBI GenBank Nucleotide #: NM_000582.2
• UniProt Accession #: P10451
• Molecular Weight: 55866.6
• NCBI Official Full Name: osteopontin isoform OPN-b
• NCBI Official Synonym Full Names: secreted phosphoprotein 1
• NCBI Official Symbol: SPP1
• NCBI Official Synonym Symbols: OPN; BNSP; BSPI; ETA-1
• NCBI Protein Information: osteopontin
• UniProt Protein Name: Osteopontin
• Protein Family: Sucrose-phosphatase
• UniProt Gene Name: SPP1
• UniProt Synonym Gene Names: BNSP; OPN; SPP-1
• UniProt Entry Name: OSTP_HUMAN

NCBI Description

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: extracellular space; cell projection; perinuclear region of cytoplasm; apical part of cell; extracellular region

Molecular Function: protein binding; extracellular matrix binding; cytokine activity

Biological Process: neutrophil chemotaxis; extracellular matrix organization and biogenesis; decidualization; osteoblast differentiation; extracellular matrix disassembly; response to vitamin D; biomineral formation; response to steroid hormone stimulus; negative regulation of collateral sprouting of intact axon in response to injury; positive regulation of bone resorption; inflammatory response; cell adhesion; embryo implantation