Rabbit Spondin 2/Mindin/Differentially expressed in cancerous and non-cancerous lung cells 1 ELISA Kit | SPON2//Mindin/ DIL-1 elisa kit
Rabbit Spondin 2/Mindin/Differentially expressed in cancerous and non-cancerous lung cells 1 ELISA Kit
Gene Names
SPON2; DIL1; DIL-1; MINDIN; M-SPONDIN
Reactivity
Rabbit
Synonyms
Spondin 2/Mindin/Differentially expressed in cancerous and non-cancerous lung cells 1; Rabbit Spondin 2/Mindin/Differentially expressed in cancerous and non-cancerous lung cells 1 ELISA Kit; SPON2//Mindin/ DIL-1 elisa kit
Reactivity
Rabbit
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for SPON2//Mindin/ DIL-1 elisa kit
Principle of the assay: SPON2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SPON2 antibody and an SPON2-HRP conjugate. The assay sample and buffer are incubated together with SPON2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SPON2 concentration since SPON2 from samples and SPON2-HRP conjugate compete for the anti-SPON2 antibody binding site. Since the number of sites is limited, as more sites are occupied by SPON2 from the sample, fewer sites are left to bind SPON2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SPON2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for SPON2//Mindin/ DIL-1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,846 Da
NCBI Official Full Name
spondin-2
NCBI Official Synonym Full Names
spondin 2, extracellular matrix protein
NCBI Official Symbol
SPON2
NCBI Official Synonym Symbols
DIL1; DIL-1; MINDIN; M-SPONDIN
NCBI Protein Information
spondin-2; differentially expressed in cancerous and non-cancerous lung cells 1
UniProt Protein Name
Spondin-2
UniProt Gene Name
SPON2
UniProt Synonym Gene Names
DIL1; UNQ435/PRO866; DIL-1
UniProt Entry Name
SPON2_HUMAN
Uniprot Description
SPON2: Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern- recognition molecule in the ECM for microbial pathogens. Binds bacterial lipopolysaccharide (LPS).
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 4p16.3
Cellular Component: extracellular space; proteinaceous extracellular matrix
Molecular Function: lipopolysaccharide binding; metal ion binding; antigen binding
Biological Process: mast cell mediated immunity; axon guidance; induction of bacterial agglutination; innate immune response; positive regulation of interleukin-6 production; opsonization; positive regulation of tumor necrosis factor production; cell adhesion; defense response to virus; defense response to fungus
