Stage IV sporulation protein FA (spoIVFA) Recombinant Protein | spoIVFA recombinant protein
Recombinant Bacillus subtilis Stage IV sporulation protein FA (spoIVFA)
NCBI and Uniprot Product Information
Uniprot Description
Function: Implicated in the coupling of mother cell to forespore gene expression. Required for spore formation at 37 degrees Celsius, but not at 30 degrees Celsius. SpoIVFA plays a central role in both maintaining the SpoIVFA/BofA/SpoIVFB complex and anchoring it to the outer forespore membrane. SpoIVFA brings BofA into close proximity to SpoIVFB, allowing BofA to inhibit SpoIVFB. Increased accumulation of SpoIVFA seems to inhibit the activity of SpoIVFB and thus regulates the activation of sigma-K.
Subunit structure: Forms a complex with BofA and SpoIVFB localized in the mother-cell membrane surrounding the forespore. Ref.5
Subcellular location: Forespore outer membrane.
Developmental stage: Transcribed during the stage II, but not required until stage IV of sporulation.
Post-translational modification: May be degraded by FtsH. It is stabilized by an ftsH disruption mutant, and in a probably independent fashion, by overexpression of BofA.
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Product Notes
The spoIVFA spoivfa (Catalog #AAA1191091) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-264. The amino acid sequence is listed below: MSHRADEIRK RLEKRRKQLS GSKRFSTQTV SEKQKPPSWV MVTDQEKHGT LPVYEDNMPT FNGKHPLVKT DSIILKCLLS ACLVLVSAIA YKTNIGPVSQ IKPAVAKTFE TEFQFASASH WFETKFGNPL AFLAPEHKNK EQQIEVGKDL IAPASGKVQQ DFQDNGEGIK VETSSDKIDS VKEGYVVEVS KDSQTGLTVK VQHADNTYSI YGELKDVDVA LYDFVDKGKK LGSIKLDDHN KGVYYFAMKD GDKFIDPIQV ISFE. It is sometimes possible for the material contained within the vial of "Stage IV sporulation protein FA (spoIVFA), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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