SPOCK1 recombinant protein

SPOCK1 Protein, Human, Recombinant (aa 21-429, His Tag)

Cat. Num. AAA8120395

• Gene Names: SPOCK1; TIC1; SPOCK; TESTICAN
• Purity: >85% as determined by SDS-PAGE
• Synonyms: SPOCK1; SPOCK1 Protein; Human; Recombinant (aa 21-429; His Tag); Human SPOCK1/Testican 1 Protein (aa 21-429; SPOCK Protein; TESTICAN Protein; TIC1 Protein; sparc/osteonectin; cwcv and kazal-like domains proteoglycan (testican) 1; SPOCK1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Trp439

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Arg 22
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human SPOCK1 (Q08629)(Met 1-Trp439) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for SPOCK1 recombinant protein

Background: Osteonectin, also known as SPOCK1, is an extracellular heparan/chondroitin sulfate proteoglycan. Members of this family are known as testicans, also called SPOCKs. They are characterized structurally by an N-terminal testican-specific domain, a follistatin-like region, a calcium-binding domain, a thyroglobulin-like domain, and an acidic C-terminal domain with two putative glycosaminoglycan attachment sites. SPOCKs are enriched in brain and have been shown to regulate neuronal attachment and outgrowth. They contain inhibitory regions in several domains targeted to different classes of protease, and in some cases may act as protease inhibitors. Osteonectin contains 1 Kazal-like domain and 1 thyroglobulin type-1 domain. Up to now, little is known about osteonectin's function. It may play a role in cell-cell and cell-matrix interactions. Osteonectin also may contribute to various neuronal mechanisms in the central nervous system.

References

Alliel P.M., et al.,(1993), Testican, a multidomain testicular proteoglycan resembling modulators of cell social behaviour. Eur. J. Biochem. 214:347-350.Marr H.S., et al., (1997), Endothelial cell expression of testican mRNA.Endothelium 5:209-219.Ota T., et al.,(2004), Complete sequencing and characterization of 21,243 full-length human cDNAs.Nat. Genet. 36:40-45.

NCBI and Uniprot Product Information

• NCBI GI #: 4759164
• NCBI GeneID: 6695
• NCBI Accession #: NP_004589.1
• NCBI GenBank Nucleotide #: NM_004598.3
• UniProt Accession #: Q08629; Q59EW0; Q8N630; Q9UCL8; B3KSW3
• Molecular Weight: 47.8 kDa
• NCBI Official Full Name: testican-1
• NCBI Official Synonym Full Names: SPARC/osteonectin, cwcv and kazal like domains proteoglycan 1
• NCBI Official Symbol: SPOCK1
• NCBI Official Synonym Symbols: TIC1; SPOCK; TESTICAN
• NCBI Protein Information: testican-1
• UniProt Protein Name: Testican-1
• Protein Family: Testican
• UniProt Gene Name: SPOCK1
• UniProt Synonym Gene Names: SPOCK; TIC1; TICN1

NCBI Description

This gene encodes the protein core of a seminal plasma proteoglycan containing chondroitin- and heparan-sulfate chains. The protein's function is unknown, although similarity to thyropin-type cysteine protease-inhibitors suggests its function may be related to protease inhibition. [provided by RefSeq, Jul 2008]

Uniprot Description

SPOCK1: May play a role in cell-cell and cell-matrix interactions. May contribute to various neuronal mechanisms in the central nervous system.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: cytoplasm; extracellular space; neuromuscular junction; sarcoplasm

Molecular Function: calcium ion binding; cysteine protease inhibitor activity; metalloendopeptidase inhibitor activity

Biological Process: central nervous system neuron differentiation; neurogenesis; neuron migration