Human serine peptidase inhibitor, Kazal type 6 ELISA Kit | SPINK6 elisa kit

Human Serine protease inhibitor Kazal-type 6, SPINK6 ELISA Kit

Cat. Num. AAA934006

• Gene Names: SPINK6; BUSI2; UNQ844
• Reactivity: Human
• Synonyms: serine peptidase inhibitor; Kazal type 6; Human Serine protease inhibitor Kazal-type 6; SPINK6 ELISA Kit; Human Serine protease inhibitor Kazal-type 6 (SPINK6) ELISA kit; BUSI2; MGC21394; UNQ844; acrosin inhibitor; protease inhibitor H; serine protease inhibitor; SPINK6 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse SP-D. No significant cross-reactivity or interference between mouse SP-D and analogues was observed.
• Sequence Length: 80

• Samples: Serum, Plasma, Cell Culture Supernates, Tissue Homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 1.56 ng/ml-100 ng/ml
• Sensitivity: 0.39 ng/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SPINK6 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SP-D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SP-D present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SP-D is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SP-D bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 305632839
• NCBI GeneID: 404203
• NCBI Accession #: NP_001182219.1
• NCBI GenBank Nucleotide #: NM_001195290.1
• UniProt Accession #: Q6UWN8; Q8N5P0; E0X656
• Molecular Weight: 8,585 Da
• NCBI Official Full Name: serine protease inhibitor Kazal-type 6
• NCBI Official Synonym Full Names: serine peptidase inhibitor, Kazal type 6
• NCBI Official Symbol: SPINK6
• NCBI Official Synonym Symbols: BUSI2; UNQ844
• NCBI Protein Information: serine protease inhibitor Kazal-type 6; acrosin inhibitor; kallikrein inhibitor; protease inhibitor H
• UniProt Protein Name: Serine protease inhibitor Kazal-type 6
• Protein Family: Serine protease inhibitor
• UniProt Gene Name: SPINK6
• UniProt Synonym Gene Names: UNQ844/PRO1782
• UniProt Entry Name: ISK6_HUMAN

NCBI Description

The protein encoded by this gene is a Kazal-type serine protease inhibitor that acts on kallikrein-related peptidases in the skin. Two transcript variants the same protein have been found for this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

SPINK6: Serine protease inhibitor selective for kallikreins. Efficiently inhibits KLK4, KLK5, KLK6, KLK7, KLK12, KLK13 and KLK14. Doesn't inhibit KLK8.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity