Rabbit anti-Human IL28B Polyclonal Antibody | anti-IL28B antibody

IL28B, NT (IL28B, IFNL3, IFNL4, IL28C, ZCYTO22, Interleukin-28B, Cytokine Zcyto22, Interferon lambda-3, Interferon lambda-4, Interleukin-28C) (Biotin)

Cat. Num. AAA6310181

• Gene Names: IFNL3; IL28B; IL28C; IL-28B
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IL28B; Polyclonal Antibody; NT (IL28B; IFNL3; IFNL4; IL28C; ZCYTO22; Interleukin-28B; Cytokine Zcyto22; Interferon lambda-3; Interferon lambda-4; Interleukin-28C) (Biotin); Interleukin-28C; anti-IL28B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-IL28B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: IL28B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-63 amino acids from the N-terminal region of human IL28B.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(IL28B Antibody (N-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the IL28B antibody detected the IL28B protein (arrow).)

Western Blot (WB)

(Western blot analysis of IL28B (arrow) using rabbit polyclonal IL28B Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the IL28B gene.)

Related Product Information for anti-IL28B antibody

This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28A (IL28A), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA).

Product Categories/Family for anti-IL28B antibody

Antibodies; Interferon

References

Lindh, M., et al. Hepatology 52(5):1860-1861(2010). Pineda, J.A., et al. Clin. Infect. Dis. 51(7):788-795(2010). Grebely, J., et al. Hepatology 52(4):1216-1224(2010). Stattermayer, A.F., et al. Clin. Gastroenterol. Hepatol. (2010). In press: Iwata, R., et al. J. Viral Hepat. (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 28144901
• NCBI GeneID: 282617
• NCBI Accession #: NP_742151.2
• NCBI GenBank Nucleotide #: NM_172139.2
• UniProt Accession #: Q8IZI9; Q6VN56; Q7Z4J3; Q8IWL6; A2BDE1
• Molecular Weight: 21,706 Da
• NCBI Official Full Name: interferon lambda-3
• NCBI Official Synonym Full Names: interferon, lambda 3
• NCBI Official Symbol: IFNL3
• NCBI Official Synonym Symbols: IL28B; IL28C; IL-28B
• NCBI Protein Information: interferon lambda-3; IL-28C; IFN-lambda-3; IFN-lambda-4; interleukin 28C; interleukin-28B; interleukin-28C; cytokine Zcyto22; interferon lambda-4; interferon, lambda 4; interleukin 28B (interferon, lambda 3)
• UniProt Protein Name: Interferon lambda-3
• UniProt Gene Name: IFNL3
• UniProt Synonym Gene Names: IL28B; IL28C; ZCYTO22; IFN-lambda-3; IL-28B; IL-28C
• UniProt Entry Name: IFNL3_HUMAN

NCBI Description

This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28A (IL28A), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). [provided by RefSeq, Jul 2008]

Uniprot Description

IL-28B: Cytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. Belongs to the IL-28/IL-29 family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.13

Cellular Component: extracellular space

Molecular Function: cytokine activity

Biological Process: positive regulation of immune response; negative regulation of viral genome replication; JAK-STAT cascade; defense response to virus

Disease: Hepatitis C Virus, Susceptibility To