Goat Pulmonary Surfactant Associated Protein C ELISA Kit | SPC elisa kit

Goat Pulmonary Surfactant Associated Protein C ELISA Kit

Cat. Num. AAA737045

• Gene Names: Sftpc; SP5; SP-C; Sftp2; Sftp-2; pro-SpC
• Reactivity: Goat
• Synonyms: Pulmonary Surfactant Associated Protein C; Goat Pulmonary Surfactant Associated Protein C ELISA Kit; SPC elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This SPC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat SPC. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SPC elisa kit

Principle of the assay: SPC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SPC antibody and an SPC-HRP conjugate. The assay sample and buffer are incubated together with SPC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SPC concentration since SPC from samples and SPC-HRP conjugate compete for the anti-SPC antibody binding site. Since the number of sites is limited, as more sites are occupied by SPC from the sample, fewer sites are left to bind SPC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SPC concentration in each sample is interpolated from this standard curve.

Product Categories/Family for SPC elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 256355065
• NCBI GeneID: 20389
• NCBI Accession #: NP_035489.2
• NCBI GenBank Nucleotide #: NM_011359.2
• UniProt Accession #: P21841; Q6P8P8
• Molecular Weight: 21,055 Da
• NCBI Official Full Name: pulmonary surfactant-associated protein C
• NCBI Official Synonym Full Names: surfactant associated protein C
• NCBI Official Symbol: Sftpc
• NCBI Official Synonym Symbols: SP5; SP-C; Sftp2; Sftp-2; pro-SpC
• NCBI Protein Information: pulmonary surfactant-associated protein C; pulmonary surfactant-associated proteolipid SPL(Val)
• UniProt Protein Name: Pulmonary surfactant-associated protein C
• UniProt Gene Name: Sftpc
• UniProt Synonym Gene Names: Sftp2; SP-C
• UniProt Entry Name: PSPC_MOUSE

Uniprot Description

SFTPC: Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air- liquid interface in the peripheral air spaces. Defects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2); also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. A rare disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course, due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress. Genetic variations in SFTPC are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS); also known as RDS in prematurity. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid-binding

Cellular Component: multivesicular body; extracellular space; cytoplasm; extracellular region

Molecular Function: protein homodimerization activity

Biological Process: protein homooligomerization