Secreted Protein Acidic & Rich in Cysteine Recombinant Protein | SPARC recombinant protein
Recombinant Human Secreted Protein Acidic & Rich in Cysteine
Sterile Filtered White lyophilized (freeze-dried) powder.
Introduction: SPARC, an acronym for œsecreted protein, acidic and rich in cysteine, is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid, 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading and proliferation, and promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. [provided by RefSeq, Dec 2011]
Uniprot Description
SPARC: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. Belongs to the SPARC family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 5q31.3-q32
Cellular Component: extracellular space; platelet alpha granule membrane; nuclear matrix; cell surface; mitochondrion; cytoplasm; plasma membrane; extracellular region; basement membrane
Molecular Function: collagen binding; protein binding; extracellular matrix binding; calcium ion binding
Biological Process: response to gravity; response to peptide hormone stimulus; receptor-mediated endocytosis; platelet activation; extracellular matrix organization and biogenesis; ossification; response to cAMP; heart development; response to glucocorticoid stimulus; response to lipopolysaccharide; signal transduction; response to L-ascorbic acid; negative regulation of angiogenesis; platelet degranulation; response to cadmium ion; response to ethanol; response to cytokine stimulus; negative regulation of endothelial cell proliferation; response to lead ion; regulation of cell morphogenesis; blood coagulation; response to calcium ion; inner ear development; lung development
Disease: Osteogenesis Imperfecta, Type Xvii
Research Articles on SPARC
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Product Notes
The SPARC sparc (Catalog #AAA143252) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MSYYHHHHHH PQQEAL PDETEVVEET VAEVTEVSVG ANPVQVEVGE FDDGAEETEE EVVAENPCQN HHCKHGKVCE LDENNTPMCV CQDPTSCPAP IGEFEKVCSN DNKTFDSSCH FFATKCTLEG TKKGHKLHLD YIGPCKYIPP CLDSELTEFP LRMRDWLKNV LVTLYERDED NNLLTKQKLR VKKIHENEKR LEAGDHPVEL LARDFEKNYN MYIFPVHWQF GQLDQHPIDG YLSHTELAPL RAPLIPMEHC TTRFFETCDL DNDKYIALDE WAGCFGIKQK DIDKDLVI. It is sometimes possible for the material contained within the vial of "Secreted Protein Acidic & Rich in Cysteine, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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