Human superoxide dismutase 3, extracellular ELISA Kit | SOD3 elisa kit

Human Extracellular superoxide dismutase [Cu-Zn], SOD3 ELISA Kit

Cat. Num. AAA936860

• Gene Names: SOD3; EC-SOD
• Reactivity: Human
• Synonyms: superoxide dismutase 3; extracellular; Human Extracellular superoxide dismutase [Cu-Zn]; SOD3 ELISA Kit; Human Extracellular superoxide dismutase [Cu-Zn] (SOD3) ELISA kit; EC-SOD; MGC20077; ; SOD3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human SOD3. No significant cross-reactivity or interference between human SOD3 and analogues was observed.
• Sequence Length: 240

• Samples: Serum, plasma, cell culture supernates, saliva, urine, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 7.8 pg/ml-500 pg/ml
• Sensitivity: < 1.95 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SOD3 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SOD3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SOD3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SOD3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SOD3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 118582275
• NCBI GeneID: 6649
• NCBI Accession #: NP_003093.2
• NCBI GenBank Nucleotide #: NM_003102.2
• UniProt Accession #: P08294; Q5U781; Q6FHA2
• Molecular Weight: 25,851 Da
• NCBI Official Full Name: extracellular superoxide dismutase
• NCBI Official Synonym Full Names: superoxide dismutase 3, extracellular
• NCBI Official Symbol: SOD3
• NCBI Official Synonym Symbols: EC-SOD
• NCBI Protein Information: extracellular superoxide dismutase [Cu-Zn]; extracellular superoxide dismutase [Cu-Zn]
• UniProt Protein Name: Extracellular superoxide dismutase [Cu-Zn]
• Protein Family: Extracellular superoxide dismutase
• UniProt Gene Name: SOD3
• UniProt Synonym Gene Names: EC-SOD
• UniProt Entry Name: SODE_HUMAN

NCBI Description

This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. The product of this gene is thought to protect the brain, lungs, and other tissues from oxidative stress. The protein is secreted into the extracellular space and forms a glycosylated homotetramer that is anchored to the extracellular matrix (ECM) and cell surfaces through an interaction with heparan sulfate proteoglycan and collagen. A fraction of the protein is cleaved near the C-terminus before secretion to generate circulating tetramers that do not interact with the ECM. [provided by RefSeq, Jul 2008]

Uniprot Description

SOD3: Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. Belongs to the Cu-Zn superoxide dismutase family.

Protein type: Oxidoreductase; Secreted; Secreted, signal peptide; EC 1.15.1.1

Chromosomal Location of Human Ortholog: 4p15.2

Cellular Component: extracellular matrix; extracellular space; Golgi lumen; cytoplasm; extracellular region; trans-Golgi network; nucleus

Molecular Function: heparin binding; protein binding; copper ion binding; zinc ion binding; superoxide dismutase activity

Biological Process: response to reactive oxygen species; removal of superoxide radicals; response to copper ion; response to hypoxia