Mouse Superoxide Dismutase 2, Mitochondrial ELISA Kit | SOD2 elisa kit

Mouse Superoxide Dismutase 2, Mitochondrial ELISA Kit

Cat. Num. AAA723112

• Gene Names: SOD2; IPOB; MNSOD; MVCD6
• Reactivity: Mouse
• Synonyms: Superoxide Dismutase 2; Mitochondrial; Mouse Superoxide Dismutase 2; Mitochondrial ELISA Kit; SOD2 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 100-2500pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This SOD-2m ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SOD-2m. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SOD2 elisa kit

Principle of the assay: SOD-2m ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SOD-2m antibody and an SOD-2m-HRP conjugate. The assay sample and buffer are incubated together with SOD-2m-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SOD-2m concentration since SOD-2m from samples and SOD-2m-HRP conjugate compete for the anti-SOD-2m antibody binding site. Since the number of sites is limited, as more sites are occupied by SOD-2m from the sample, fewer sites are left to bind SOD-2m-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SOD-2m concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 15214595
• NCBI GeneID: 6648
• Molecular Weight: 19,730 Da
• NCBI Official Full Name: Superoxide dismutase 2, mitochondrial
• NCBI Official Synonym Full Names: superoxide dismutase 2, mitochondrial
• NCBI Official Symbol: SOD2
• NCBI Official Synonym Symbols: IPOB; MNSOD; MVCD6
• NCBI Protein Information: superoxide dismutase [Mn], mitochondrial; superoxide dismutase [Mn], mitochondrial; indophenoloxidase B; Mn superoxide dismutase; mangano-superoxide dismutase; manganese-containing superoxide dismutase
• UniProt Protein Name: Superoxide dismutase [Mn], mitochondrial
• Protein Family: Superoxide dismutase
• UniProt Gene Name: SOD2
• UniProt Entry Name: SODM_HUMAN

NCBI Description

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

SOD2: Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the iron/manganese superoxide dismutase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.15.1.1; Oxidoreductase; Mitochondrial

Chromosomal Location of Human Ortholog: 6q25.3

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane

Molecular Function: identical protein binding; DNA binding; manganese ion binding; superoxide dismutase activity; oxygen binding

Biological Process: oxygen homeostasis; positive regulation of nitric oxide biosynthetic process; removal of superoxide radicals; heart development; locomotory behavior; response to lipopolysaccharide; response to L-ascorbic acid; post-embryonic development; protein homotetramerization; negative regulation of cell proliferation; response to selenium ion; glutathione metabolic process; regulation of mitochondrial membrane potential; acetylcholine vasodilation involved in regulation of systemic arterial blood pressure; regulation of catalytic activity; regulation of blood pressure; response to gamma radiation; hemopoiesis; negative regulation of neuron apoptosis; response to axon injury; response to electrical stimulus; response to drug; erythrophore differentiation; release of cytochrome c from mitochondria; response to superoxide; superoxide metabolic process; negative regulation of fat cell differentiation; liver development; regulation of transcription from RNA polymerase II promoter; response to manganese ion; response to silicon dioxide; response to reactive oxygen species; iron ion homeostasis; response to cadmium ion; response to hyperoxia; response to hydrogen peroxide; DNA damage response, signal transduction resulting in induction of apoptosis; age-dependent response to reactive oxygen species; detection of oxygen; negative regulation of fibroblast proliferation; response to zinc ion; response to hypoxia; neuron development; response to cold; response to activity; induction of apoptosis by oxidative stress; superoxide release; hydrogen peroxide biosynthetic process

Disease: Microvascular Complications Of Diabetes, Susceptibility To, 6