Rabbit RPS18 Polyclonal Antibody | anti-RPS18 antibody

RPS18 Polyclonal Antibody

Cat. Num. AAA3006171

• Gene Names: RPS18; KE3; S18; HKE3; KE-3; D6S218E
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RPS18; Polyclonal Antibody; RPS18 Polyclonal Antibody; 40S ribosomal protein S18; Ke-3; Ke3; D6S218E; anti-RPS18 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RPS18 polyclonal antibody detects endogenous levels of RPS18 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 152

• Applicable Applications for anti-RPS18 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 107-152 of Human RPS18.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RPS18 polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:Raw264. 7 cell lysateLane3:H9C2 cell lysate)

Related Product Information for anti-RPS18 antibody

Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins. Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein S18, also designated 40S ribosomal protein S18, RPS18, D6S218E, KE3 or HKE3, is a 152 amino acid cytoplasmic protein belonging to the ribosomal protein S13P family. A component of the 40S subunit, Ribosomal Protein S18 is a known binding partner of Cofilin and has been suggested to be a novel substrate for CaMKII. The gene encoding Ribosomal Protein S18 maps to human chromosome 6p21.32, and like most ribosomal proteins, Ribosomal Protein S18 exists as multiple processed pseudogenes that are scattered throughout the genome.

NCBI and Uniprot Product Information

• NCBI GI #: 11968182
• NCBI GeneID: 6222
• NCBI Accession #: NP_072045.1
• NCBI GenBank Nucleotide #: NP_072045.1
• UniProt Accession #: P62269
• Molecular Weight: ~ 18kDa
• NCBI Official Full Name: 40S ribosomal protein S18
• NCBI Official Synonym Full Names: ribosomal protein S18
• NCBI Official Symbol: RPS18
• NCBI Official Synonym Symbols: KE3; S18; HKE3; KE-3; D6S218E
• NCBI Protein Information: 40S ribosomal protein S18
• UniProt Protein Name: 40S ribosomal protein S18
• Protein Family: 40S ribosomal protein
• UniProt Gene Name: RPS18
• UniProt Synonym Gene Names: D6S218E; Ke3
• UniProt Entry Name: RS18_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S13P family of ribosomal proteins. It is located in the cytoplasm. The gene product of the E. coli ortholog (ribosomal protein S13) is involved in the binding of fMet-tRNA, and thus, in the initiation of translation. This gene is an ortholog of mouse Ke3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPS18: Located at the top of the head of the 40S subunit, it contacts several helices of the 18S rRNA. Belongs to the ribosomal protein S13P family.

Protein type: Translation; Ribosomal

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: small ribosomal subunit; focal adhesion; membrane; cytoplasm; ribosome; nucleus; cytosol

Molecular Function: rRNA binding; protein binding; structural constituent of ribosome

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; translation; viral reproduction; mRNA catabolic process, nonsense-mediated decay; translational initiation; ribosome biogenesis and assembly; viral transcription; gene expression; viral infectious cycle; translational termination