SNAP25B Recombinant Protein | SNAP25 recombinant protein

SNAP25B, Recombinant, Human (Synaptosomal-associated protein 25-binding protein)

Cat. Num. AAA623360

• Gene Names: SNAP25; RIC4; SEC9; SNAP; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
• Applications: ELISA
• Purity: > 90% by SDS PAGE
• Synonyms: SNAP25B; Recombinant; Human (Synaptosomal-associated protein 25-binding protein); Anti -SNAP25B; SNAP25 recombinant protein

• Purity/Purification: > 90% by SDS PAGE
• Form/Format: Supplied as a liquid in 25mM Tris pH 7.5, 1mM DTT, 2mM EDTA, 10% glycerol.
• Sequence: MAEDADMRNE LEEMQRRADQ LADESLESTR RMLQLVEESK DAGIRTLVML; DEQGEQLERI EEGMDQINKD MKEAEKNLTD LGKFCGLCVC PCNKLKSSDA YKKAWGNNQD; GVVASQPARV VDEREQMAIS GGFIRRVTND ARENEMDENL EQVSGIIGNL RHMALDMGNE; IDTQNRQIDR IMEKADSNKT RIDEANQRAT KMLGSG

• Applicable Applications for SNAP25 recombinant protein: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

SDS-Page

(15% SDS-PAGE(3μg))

Related Product Information for SNAP25 recombinant protein

The synaptosomal-associated protein (SNAP-25) is an essential component of the core complex that mediates presynaptic vesicle trafficking. Thus, SNAP-25 is directly involved in the release of neurotransmitters and this protein exists as two alternative isoforms, SNAP25A and SNAP25B which differ by 9 amino acids in central portion of these proteins.

Product Categories/Family for SNAP25 recombinant protein

Antibodies; Abs to Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 18765735
• NCBI GeneID: 6616
• NCBI Accession #: NP_570824.1
• NCBI GenBank Nucleotide #: NM_130811.2
• UniProt Accession #: P60880; P13795; P36974; P70557; P70558; Q53EM2; Q5U0B5; Q8IXK3; Q96FM2; B2RAU4; D3DW16; D3DW17
• Molecular Weight: 23kD (206 aa)
• NCBI Official Full Name: synaptosomal-associated protein 25 isoform SNAP25B
• NCBI Official Synonym Full Names: synaptosomal-associated protein, 25kDa
• NCBI Official Symbol: SNAP25
• NCBI Official Synonym Symbols: RIC4; SEC9; SNAP; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
• NCBI Protein Information: synaptosomal-associated protein 25; SUP; super protein; resistance to inhibitors of cholinesterase 4 homolog
• UniProt Protein Name: Synaptosomal-associated protein 25
• Protein Family: Synaptosomal-associated protein
• UniProt Gene Name: SNAP25
• UniProt Synonym Gene Names: SNAP; SNAP-25; SUP
• UniProt Entry Name: SNP25_HUMAN

NCBI Description

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SNAP-25: a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Part of the SNARE core complex containing SNAP25, VAMP2 and syntaxin 1A. Associates with proteins involved in vesicle docking and membrane fusion. Two alternatively spliced isoforms have been described.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 20p12-p11.2

Cellular Component: SNARE complex; presynaptic membrane; neuron projection; growth cone; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; trans-Golgi network; cytosol; cell junction; vesicle

Molecular Function: SNAP receptor activity; protein binding; syntaxin-1 binding; calcium-dependent protein binding

Biological Process: synaptic vesicle fusion to presynaptic membrane; synaptic transmission; synaptic vesicle exocytosis; glutamate secretion; neurotransmitter secretion; energy reserve metabolic process; neurotransmitter uptake; pathogenesis; synaptic vesicle priming; regulation of insulin secretion; synaptic vesicle docking during exocytosis

Disease: Myasthenic Syndrome, Congenital, 18