SMuG1 recombinant protein

SMuG1, 1-270aa, Human, His tag, E Coli

Cat. Num. AAA205279

• Gene Names: SMUG1; FDG; UNG3; HMUDG
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: SMuG1; 1-270aa; Human; His tag; E Coli; Single-strand-selective monofunctional uracil-DNA glycosylase; FDG; HMuDG; uNG3; SMuG1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 30% glycerol, 1mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPQAFLL GSIHEPAGAL MEPQPCPGSL AESFLEEELR LNAELSQLQF SEPVGIIYNP VEYAWEPHRN YVTRYCQGPK EVLFLGMNPG PFGMAQTGVP FGEVSMVRDW LGIVGPVLTP PQEHPKRPVL GLECPQSEVS GARFWGFFRN LCGQPEVFFH HCFVHNLCPL LFLAPSGRNL TPAELPAKQR EQLLGICDAA LCRQVQLLGV RLVVGVGRLA EQRARRALAG LMPEVQVEGL LHPSPRNPQA NKGWEAVAKE RLNELGLLPL LLK
• Sequence Length: 270

• Applicable Applications for SMuG1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for SMuG1 recombinant protein

Single-strand-selective monofunctional uracil-DNA glycosylase, also known as SMuG1, is a enzyme responsible for recognizing base lesions in the genome and initiating base excision DNA repair. This protein acts as a monofunctional DNA glycosylase specific for uracil (u) residues in DNA and has a preference for single-stranded DNA substrates. Recombinant human SMuG1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for SMuG1 recombinant protein

Epigenomics

References

Masaoka A., et al. (2003) Biochemistry. 42:5003-5012; Boorstein R.J., et al. (2001) J. Biol. Chem. 276:41991-41997;

NCBI and Uniprot Product Information

• NCBI GI #: 7657597
• NCBI GeneID: 23583
• NCBI Accession #: NP_055126
• NCBI GenBank Nucleotide #: NM_014311.2
• UniProt Accession #: Q53HV7; O95862; Q0D2M0; Q8NB71; Q9BWC8; A8K2K9
• Molecular Weight: 32.3 kDa (293aa), confirmed by MALDI-TOF
• NCBI Official Full Name: single-strand selective monofunctional uracil DNA glycosylase isoform 1
• NCBI Official Synonym Full Names: single-strand-selective monofunctional uracil-DNA glycosylase 1
• NCBI Official Symbol: SMUG1
• NCBI Official Synonym Symbols: FDG; UNG3; HMUDG
• NCBI Protein Information: single-strand selective monofunctional uracil DNA glycosylase
• UniProt Protein Name: Single-strand selective monofunctional uracil DNA glycosylase
• Protein Family: Single-strand selective monofunctional uracil DNA glycosylase
• UniProt Gene Name: SMUG1

NCBI Description

This gene encodes a protein that participates in base excision repair by removing uracil from single- and double-stranded DNA. Many alternatively spliced transcript variants exist for this gene; the full-length nature is known for some but not all of the variants. [provided by RefSeq, Aug 2011]

Uniprot Description

Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration.