Human Soluble Mesothelin Related Peptide ELISA Kit | SMRP elisa kit

Human Soluble Mesothelin Related Peptide ELISA Kit

Cat. Num. AAA760044

• Gene Names: ABCC5; MRP5; SMRP; ABC33; MOATC; MOAT-C; pABC11; EST277145
• Reactivity: Human
• Synonyms: Soluble Mesothelin Related Peptide; Human Soluble Mesothelin Related Peptide ELISA Kit; SMRP/Soluble Mesothelin Related Peptide; SMRP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SMRP. No significant cross-reactivity or interference between SMRP and analogues was observed.
• Sequence Length: 946

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20pmol/ml
• Sensitivity: 0.188pmol/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SMRP elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 2554610
• NCBI GeneID: 10057
• Molecular Weight: 155,732 Da
• NCBI Official Full Name: SMRP
• NCBI Official Synonym Full Names: ATP binding cassette subfamily C member 5
• NCBI Official Symbol: ABCC5
• NCBI Official Synonym Symbols: MRP5; SMRP; ABC33; MOATC; MOAT-C; pABC11; EST277145
• NCBI Protein Information: multidrug resistance-associated protein 5
• UniProt Protein Name: Multidrug resistance-associated protein 5
• UniProt Gene Name: ABCC5
• UniProt Synonym Gene Names: MRP5; MOAT-C
• UniProt Entry Name: MRP5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions in the cellular export of its substrate, cyclic nucleotides. This export contributes to the degradation of phosphodiesterases and possibly an elimination pathway for cyclic nucleotides. Studies show that this protein provides resistance to thiopurine anticancer drugs, 6-mercatopurine and thioguanine, and the anti-HIV drug 9-(2-phosphonylmethoxyethyl)adenine. This protein may be involved in resistance to thiopurines in acute lymphoblastic leukemia and antiretroviral nucleoside analogs in HIV-infected patients. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Uniprot Description

ABCC5: Acts as a multispecific organic anion pump which can transport nucleotide analogs. Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, ABC family; Membrane protein, integral; Membrane protein, multi-pass; Transporter

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: integral to plasma membrane; membrane; plasma membrane

Molecular Function: ATP binding; ATPase activity, coupled to transmembrane movement of substances; organic anion transmembrane transporter activity

Biological Process: carbohydrate metabolic process; glycosaminoglycan metabolic process; hyaluronan biosynthetic process; hyaluronan metabolic process; organic anion transport; transmembrane transport; transport