Human ABCC5 ELISA Kit | ABCC5 elisa kit

Human ABCC5 (ATP Binding Cassette Transporter C5)ELISA Kit

Cat. Num. AAA2515792

• Gene Names: ABCC5; MRP5; SMRP; ABC33; MOATC; MOAT-C; pABC11; EST277145
• Reactivity: Human
• Synonyms: ABCC5; Human ABCC5 (ATP Binding Cassette Transporter C5)ELISA Kit; ABCC5 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinantHumanABCC5. No significant cross-reactivity or interference between HumanABCC5 and analogues was observed.
• Sequence Length: 225

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 78.125-5000pg/mL
• Sensitivity: Min: 46.875pg/mL; Max: 5000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ABCC5 elisa kit

Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanABCC5concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ABCC5. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ABCC5and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ABCC5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ABCC5.You can calculate the concentration of ABCC5in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 58866242
• NCBI GeneID: 10057
• Molecular Weight: 155,732 Da
• NCBI Official Full Name: ABCC5 variant 4
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family C (CFTR/MRP), member 5
• NCBI Official Symbol: ABCC5
• NCBI Official Synonym Symbols: MRP5; SMRP; ABC33; MOATC; MOAT-C; pABC11; EST277145
• NCBI Protein Information: multidrug resistance-associated protein 5; ATP-binding cassette sub-family C member 5; canalicular multispecific organic anion transporter C; multi-specific organic anion transporter C
• UniProt Protein Name: Multidrug resistance-associated protein 5
• Protein Family: ABC transporter C family
• UniProt Gene Name: ABCC5
• UniProt Synonym Gene Names: MRP5; MOAT-C
• UniProt Entry Name: MRP5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions in the cellular export of its substrate, cyclic nucleotides. This export contributes to the degradation of phosphodiesterases and possibly an elimination pathway for cyclic nucleotides. Studies show that this protein provides resistance to thiopurine anticancer drugs, 6-mercatopurine and thioguanine, and the anti-HIV drug 9-(2-phosphonylmethoxyethyl)adenine. This protein may be involved in resistance to thiopurines in acute lymphoblastic leukemia and antiretroviral nucleoside analogs in HIV-infected patients. Alternative splicing of this gene has been detected; however, the complete sequence and translation initiation site is unclear. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCC5: Acts as a multispecific organic anion pump which can transport nucleotide analogs. Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter, ABC family; Transporter

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: membrane; integral to plasma membrane; plasma membrane

Molecular Function: ATPase activity, coupled to transmembrane movement of substances; organic anion transmembrane transporter activity; ATP binding

Biological Process: glycosaminoglycan metabolic process; transport; hyaluronan biosynthetic process; carbohydrate metabolic process; pathogenesis; hyaluronan metabolic process; transmembrane transport; organic anion transport