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Mouse Spermine oxidase ELISA Kit | SMOX elisa kit

Mouse Spermine oxidase (SMOX) ELISA Kit

Gene Names
Smox; PAO; SMO; PAOh1; B130066H01Rik
Reactivity
Mouse
Synonyms
Spermine oxidase; Mouse Spermine oxidase (SMOX) ELISA Kit; C20orf16; FLJ20746; MGC1010; PAO; PAOH1; SMO; OTTHUMP00000030158|flavin containing amine oxidase|flavin-containing spermine oxidase|polyamine oxidase|putative cyclin G1 interacting protein; SMOX elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse SMOX. No significant cross-reactivity or interference between Mouse SMOX and analogues was observed.
Sequence Length
555
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for SMOX elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate SMOX in samples. An antibody specific for SMOX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySMOX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMOX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMOX bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,647 Da
NCBI Official Full Name
spermine oxidase isoform a
NCBI Official Synonym Full Names
spermine oxidase
NCBI Official Symbol
Smox
NCBI Official Synonym Symbols
PAO; SMO; PAOh1; B130066H01Rik
NCBI Protein Information
spermine oxidase
UniProt Protein Name
Spermine oxidase
Protein Family
UniProt Gene Name
Smox
UniProt Synonym Gene Names
Smo; PAO-1; PAOh1

Uniprot Description

SMOX: Flavoenzyme which catalyzes the oxidation of spermine to spermidine. Can also use N(1)-acetylspermine and spermidine as substrates, with different affinity depending on the isoform (isozyme) and on the experimental conditions. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs. May contribute to beta-alanine production via aldehyde dehydrogenase conversion of 3-amino-propanal. Belongs to the flavin monoamine oxidase family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.5.3.16; Oxidoreductase

Chromosomal Location of Human Ortholog: 2|2 F1

Cellular Component: cytoplasm; intracellular membrane-bound organelle; nuclear membrane; nucleoplasm; nucleus

Molecular Function: oxidoreductase activity; polyamine oxidase activity

Biological Process: polyamine catabolic process; spermine catabolic process

Research Articles on SMOX

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Product Notes

The Mouse SMOX smox (Catalog #AAA285841) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA285841 ELISA Kit recognizes Mouse SMOX. It is sometimes possible for the material contained within the vial of "Spermine oxidase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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