Rabbit anti-Human, Mouse SMNDC1 Polyclonal Antibody | anti-SMNDC1 antibody

SMNDC1, NT (SMNDC1, SMNR, SPF30, Survival of motor neuron-related-splicing factor 30, 30kD splicing factor SMNrp, SMN-related protein, Survival motor neuron domain-containing protein 1) (Biotin)

Cat. Num. AAA6348981

• Gene Names: SMNDC1; SMNR; SPF30; TDRD16C
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SMNDC1; Polyclonal Antibody; NT (SMNDC1; SMNR; SPF30; Survival of motor neuron-related-splicing factor 30; 30kD splicing factor SMNrp; SMN-related protein; Survival motor neuron domain-containing protein 1) (Biotin); Survival motor neuron domain-containing protein 1; anti-SMNDC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-SMNDC1 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SMNDC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19~48 amino acids from the N-terminal region of human SMNDC1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SMNDC1 Antibody (N-term) in mouse kidney tissue lysates (35ug/lane). SMNDC1 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(SMNDC1 Antibody (N-term) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-SMNDC1 antibody

This protein is a paralog of SMN1 gene, which encodes the survival motor neuron protein, mutations in which are cause of autosomal recessive proximal spinal muscular atrophy. The protein encoded by this gene is a nuclear protein that has been identified as a constituent of the spliceosome complex. This protein is differentially expressed, with abundant levels in skeletal muscle, and may share similar cellular function as the SMN1 gene.

Product Categories/Family for anti-SMNDC1 antibody

Antibodies; Splicing Factors

References

Little, J.T. J. Biol. Chem. 283 (13), 8145-8152 (2008). Ewing, R.M., Mol. Syst. Biol. 3, 89 (2007). Beausoleil, S.A., Nat. Biotechnol. 24 (10), 1285-1292 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 37087924
• NCBI GeneID: 10285
• UniProt Accession #: O75940; Q5T3K6; B2RA27; D3DRB1
• Molecular Weight: 238
• NCBI Official Full Name: Survival of motor neuron-related-splicing factor 30
• NCBI Official Synonym Full Names: survival motor neuron domain containing 1
• NCBI Official Symbol: SMNDC1
• NCBI Official Synonym Symbols: SMNR; SPF30; TDRD16C
• NCBI Protein Information: survival of motor neuron-related-splicing factor 30; SMN-related protein; tudor domain containing 16C; 30 kDa splicing factor SMNrp; survival motor neuron domain-containing protein 1; splicing factor 30, survival of motor neuron-related
• UniProt Protein Name: Survival of motor neuron-related-splicing factor 30
• Protein Family: Survival of motor neuron-related-splicing factor
• UniProt Gene Name: SMNDC1
• UniProt Synonym Gene Names: SMNR; SPF30
• UniProt Entry Name: SPF30_HUMAN

NCBI Description

This gene is a paralog of SMN1 gene, which encodes the survival motor neuron protein, mutations in which are cause of autosomal recessive proximal spinal muscular atrophy. The protein encoded by this gene is a nuclear protein that has been identified as a constituent of the spliceosome complex. This gene is differentially expressed, with abundant levels in skeletal muscle, and may share similar cellular function as the SMN1 gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SPF30: Necessary for spliceosome assembly. Overexpression causes apoptosis. Belongs to the SMN family.

Protein type: Spliceosome; RNA processing; RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 10q23

Cellular Component: nucleoplasm; spliceosome; Cajal body; intermediate filament cytoskeleton; cytoplasm; nuclear speck; nucleus

Molecular Function: protein binding

Biological Process: RNA splicing, via transesterification reactions; apoptosis; RNA splicing; spliceosome assembly; mRNA processing