Serine/threonine-protein kinase SMG1 (SMG1) Recombinant Protein | SMG1 recombinant protein

Recombinant Human Serine/threonine-protein kinase SMG1 (SMG1) , partial

Cat. Num. AAA1305519

• Gene Names: SMG1; ATX; LIP; 61E3.4
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Serine/threonine-protein kinase SMG1 (SMG1); Recombinant Human Serine/threonine-protein kinase SMG1 (SMG1); partial; SMG1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 2150-2478aa; Partial(PI3K/PI4K domain)
• Sequence: YPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKTVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNR

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for SMG1 recombinant protein

This gene encodes a protein involved in nonsense-mediated mRNA decay (NMD) as part of the mRNA surveillance complex. The protein has kinase activity and is thought to function in NMD by phosphorylating the regulator of nonsense transcripts 1 protein. Alternative spliced transcript variants have been described, but their full-length natures have not been determined.

NCBI and Uniprot Product Information

• NCBI GI #: 62243658
• NCBI GeneID: 23049
• NCBI Accession #: NP_055907.3
• NCBI GenBank Nucleotide #: NM_015092.4
• UniProt Accession #: Q96Q15; O43305; Q13284; Q8NFX2; Q96QV0; Q96RW3
• Molecular Weight: 269,425 Da
• NCBI Official Full Name: serine/threonine-protein kinase SMG1
• NCBI Official Synonym Full Names: SMG1, nonsense mediated mRNA decay associated PI3K related kinase
• NCBI Official Symbol: SMG1
• NCBI Official Synonym Symbols: ATX; LIP; 61E3.4
• NCBI Protein Information: serine/threonine-protein kinase SMG1
• UniProt Protein Name: Serine/threonine-protein kinase SMG1
• Protein Family: Probable serine/threonine-protein kinase
• UniProt Gene Name: SMG1
• UniProt Synonym Gene Names: ATX; KIAA0421; LIP; SMG-1; hSMG-1; Lambda-interacting protein

NCBI Description

This gene encodes a protein involved in nonsense-mediated mRNA decay (NMD) as part of the mRNA surveillance complex. The protein has kinase activity and is thought to function in NMD by phosphorylating the regulator of nonsense transcripts 1 protein. Alternatively spliced transcript variants have been described, but their full-length nature has yet to be determined. [provided by RefSeq, Mar 2013]

Uniprot Description

Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ.