Structural Maintenance Of Chromosomes Protein 3 (SMC3) Recombinant Protein | SMC3 recombinant protein

Recombinant Structural Maintenance Of Chromosomes Protein 3 (SMC3)

Cat. Num. AAA2018262

• Gene Names: SMC3; BAM; BMH; HCAP; CDLS3; CSPG6; SMC3L1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Structural Maintenance Of Chromosomes Protein 3 (SMC3); Recombinant Structural Maintenance Of Chromosomes Protein 3 (SMC3); SMC3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SEQKEKL IKRQEELDRG YKSIMELMNV LELRKYEAIQ LTFKQVSKNF SEVFQKLVPG GKATLVMKKG DVEGSQSQDE GEGSGESERG SGSQSSVPSV DQFTGVGIRV SFTGKQGEMR EMQQLSGGQK SLVALALIFAIQKCDPAPFY LFDEIDQALD AQHRKAVSDM IMELAVHAQF ITTTFRPELL E
• Sequence Length: 1217

• Applicable Applications for SMC3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Ser994~Glu1181 (Accession # Q9UQE7) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Nucleus. Chromosome. Centromere
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.1
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4885399
• NCBI GeneID: 9126
• NCBI Accession #: NP_005436.1
• NCBI GenBank Nucleotide #: NM_005445.3
• UniProt Accession #: Q9UQE7; O60464; Q5T482; A8K156
• Molecular Weight: 24.8kDa
• NCBI Official Full Name: structural maintenance of chromosomes protein 3
• NCBI Official Synonym Full Names: structural maintenance of chromosomes 3
• NCBI Official Symbol: SMC3
• NCBI Official Synonym Symbols: BAM; BMH; HCAP; CDLS3; CSPG6; SMC3L1
• NCBI Protein Information: structural maintenance of chromosomes protein 3; SMC protein 3; chromosome-associated polypeptide; chondroitin sulfate proteoglycan 6 (bamacan); basement membrane-associated chondroitin proteoglycan
• UniProt Protein Name: Structural maintenance of chromosomes protein 3
• UniProt Gene Name: SMC3
• UniProt Synonym Gene Names: BAM; BMH; CSPG6; SMC3L1; SMC protein 3; SMC-3; Bamacan; hCAP
• UniProt Entry Name: SMC3_HUMAN

NCBI Description

This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein. [provided by RefSeq, Jul 2008]

Uniprot Description

SMC3: Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement. Interacts with MXI1, MXD3 and MXD4. Interacts with SYCP2. Found in a complex with SMC1A, CDCA5 and RAD21, PDS5A/APRIN and PDS5B/SCC-112. Forms a heterodimer with SMC1A or SMC1B in cohesin complexes. Cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer attached via their hinge domain, RAD21 which link them, and one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21. Also found in meiosis-specific cohesin complexes. Interacts with NUMA1, and forms a ternary complex with KIF3B and KIFAP3, suggesting a function in tethering the chromosomes to the spindle pole and in chromosome movement. Interacts with PDS5A and WAPAL; regulated by SMC3 acetylation. Belongs to the SMC family. SMC3 subfamily.

Protein type: DNA repair, damage; Cell cycle regulation

Chromosomal Location of Human Ortholog: 10q25

Cellular Component: cohesin complex; spindle pole; cohesin core heterodimer; nuclear matrix; chromosome; cytosol; nucleoplasm; lateral element; cytoplasm; basement membrane; chromatin; nucleus; chromosome, pericentric region

Molecular Function: dynein binding; protein binding; protein heterodimerization activity; chromatin binding; microtubule motor activity; ATP binding

Biological Process: mitosis; meiosis; mitotic spindle organization and biogenesis; cell division; stem cell maintenance; mitotic sister chromatid cohesion; sister chromatid cohesion; negative regulation of DNA endoreduplication; mitotic cell cycle; signal transduction; DNA repair; regulation of DNA replication

Disease: Cornelia De Lange Syndrome 3