Mouse Mothers against decapentaplegic homolog 7 ELISA Kit | Smad7 elisa kit

Mouse Mothers against decapentaplegic homolog 7 ELISA Kit

Cat. Num. AAA763280

• Gene Names: Smad7; Madh7
• Reactivity: Mouse
• Synonyms: Mothers against decapentaplegic homolog 7; Mouse Mothers against decapentaplegic homolog 7 ELISA Kit; Smad7/MADH7 (Mothers Against Decapentaplegic Homolog 7)/MADH8/CRCS3/hSMAD7/MAD homolog 7/MAD homolog 8/MAD; mothers against decapentaplegic homolog 7 (Drosophila)/Mothers against decapentaplegic homolog 8; Smad7 elisa kit

• Reactivity: Mouse
• Sequence Length: 426

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Smad7 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Smad7 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Smad7 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Smad7 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofMouse Smad7 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 111154105
• NCBI GeneID: 17131
• NCBI Accession #: NP_001036125.1
• NCBI GenBank Nucleotide #: NM_001042660.1
• Molecular Weight: 46,371 Da
• NCBI Official Full Name: mothers against decapentaplegic homolog 7
• NCBI Official Synonym Full Names: SMAD family member 7
• NCBI Official Symbol: Smad7
• NCBI Official Synonym Symbols: Madh7
• NCBI Protein Information: mothers against decapentaplegic homolog 7
• UniProt Protein Name: Mothers against decapentaplegic homolog 7
• Protein Family: Mothers against decapentaplegic
• UniProt Gene Name: Smad7
• UniProt Synonym Gene Names: Madh7; Madh8; MAD homolog 7; Mothers against DPP homolog 7; MAD homolog 8; Mothers against DPP homolog 8; SMAD 7; Smad7
• UniProt Entry Name: SMAD7_MOUSE

Uniprot Description

SMAD7: Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A- PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. Genetic variations in SMAD7 influence susceptibility to colorectal cancer type 3 (CRCS3). Colorectal cancer consists of tumors or cancer of either the colon or rectum or both. Cancers of the large intestine are the second most common form of cancer found in males and females. Symptoms include rectal bleeding, occult blood in stools, bowel obstruction and weight loss. Treatment is based largely on the extent of cancer penetration into the intestinal wall. Surgical cures are possible if the malignancy is confined to the intestine. Risk can be reduced when following a diet which is low in fat and high in fiber. Belongs to the dwarfin/SMAD family.

Protein type: Transcription factor; DNA-binding

Cellular Component: catenin complex; cell-cell adherens junction; centrosome; cytoplasm; intracellular; nucleolus; nucleoplasm; nucleus; plasma membrane; protein complex; transcription factor complex

Molecular Function: activin binding; beta-catenin binding; collagen binding; DNA binding; metal ion binding; protein binding; transcription factor activity; transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity; ubiquitin protein ligase binding

Biological Process: artery morphogenesis; negative regulation of BMP signaling pathway; negative regulation of peptidyl-serine phosphorylation; negative regulation of protein ubiquitination; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; negative regulation of ubiquitin-protein ligase activity; positive regulation of cell-cell adhesion; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein ubiquitination; protein stabilization; regulation of activin receptor signaling pathway; regulation of transcription, DNA-dependent; regulation of transforming growth factor beta receptor signaling pathway; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; ventricular cardiac muscle morphogenesis