Monkey Mothers Against Decapentaplegic Homolog 4 ELISA Kit | Smad4 elisa kit

Monkey Mothers Against Decapentaplegic Homolog 4 ELISA Kit

Cat. Num. AAA741251

• Gene Names: SMAD4; JIP; DPC4; MADH4; MYHRS
• Reactivity: Monkey
• Synonyms: Mothers Against Decapentaplegic Homolog 4; Monkey Mothers Against Decapentaplegic Homolog 4 ELISA Kit; Smad4 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This Smad4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey Smad4. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Smad4 elisa kit

Principle of the assay: Smad4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Smad4 antibody and an Smad4-HRP conjugate. The assay sample and buffer are incubated together with Smad4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Smad4 concentration since Smad4 from samples and Smad4-HRP conjugate compete for the anti-Smad4 antibody binding site. Since the number of sites is limited, as more sites are occupied by Smad4 from the sample, fewer sites are left to bind Smad4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Smad4 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for Smad4 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 13603414
• NCBI GeneID: 4089
• Molecular Weight: 60,439 Da
• NCBI Official Full Name: SMAD4, partial
• NCBI Official Synonym Full Names: SMAD family member 4
• NCBI Official Symbol: SMAD4
• NCBI Official Synonym Symbols: JIP; DPC4; MADH4; MYHRS
• NCBI Protein Information: mothers against decapentaplegic homolog 4; MAD homolog 4; SMAD, mothers against DPP homolog 4; deleted in pancreatic carcinoma locus 4; deletion target in pancreatic carcinoma 4; mothers against decapentaplegic, Drosophila, homolog of, 4
• UniProt Protein Name: Mothers against decapentaplegic homolog 4
• Protein Family: Mothers against decapentaplegic
• UniProt Gene Name: SMAD4
• UniProt Synonym Gene Names: DPC4; MADH4; MAD homolog 4; Mothers against DPP homolog 4; SMAD 4; Smad4; hSMAD4
• UniProt Entry Name: SMAD4_HUMAN

NCBI Description

This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq, Oct 2009]

Uniprot Description

SMAD4: transcription factor that mediates signal transduction by the transforming growth factor superfamily. The common smad (co-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. Promotes binding of the Smad2/Smad4/Fast-1 complex to DNA and provides an activation function required for Smad1 or Smad2 to stimulate transcription.

Protein type: Nuclear receptor co-regulator; DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: nucleoplasm; centrosome; transcription factor complex; cytoplasm; nuclear chromatin; cytosol; nucleus

Molecular Function: collagen binding; identical protein binding; protein binding; transforming growth factor beta receptor, common-partner cytoplasmic mediator activity; protein homodimerization activity; DNA binding; sequence-specific DNA binding; metal ion binding; chromatin binding; transcription factor activity

Biological Process: axon guidance; developmental growth; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; sebaceous gland development; negative regulation of transcription from RNA polymerase II promoter; palate development; BMP signaling pathway; negative regulation of cell proliferation; regulation of transforming growth factor beta receptor signaling pathway; transforming growth factor beta receptor signaling pathway; mesoderm development; neural crest cell differentiation; positive regulation of BMP signaling pathway; transcription initiation from RNA polymerase II promoter; regulation of transforming growth factor-beta2 production; regulation of binding; transcription, DNA-dependent; in utero embryonic development; positive regulation of transforming growth factor beta receptor signaling pathway; neuron fate commitment; gastrulation with mouth forming second; somite rostral/caudal axis specification; formation of anatomical boundary; SMAD protein complex assembly; endothelial cell activation; cell proliferation; ureteric bud branching; response to hypoxia; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; regulation of hair follicle development; negative regulation of protein catabolic process; negative regulation of transcription, DNA-dependent; endoderm development

Disease: Pancreatic Cancer; Juvenile Polyposis Syndrome; Myhre Syndrome; Juvenile Polyposis/hereditary Hemorrhagic Telangiectasia Syndrome