Slit Homolog 3 (Slit3) Recombinant Protein | Slit3 recombinant protein

Recombinant Slit Homolog 3 (Slit3)

Cat. Num. AAA2009904

• Gene Names: SLIT3; MEGF5; SLIL2; SLIT1; slit2; Slit-3
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Slit Homolog 3 (Slit3); Recombinant Slit Homolog 3 (Slit3); Slit3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.; MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEF-KNDSAN ACSAFKCHHG QCHISDQGEP YCLCQPGFSG EHCQQENPCL GQVVREVIRR QKGYASCATA SKVPIMECRG GCGPQCCQPT RSKRRKYVFQ CTDGSSFVEE VE
• Sequence Length: 1530

• Applicable Applications for Slit3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Lys1405~Glu1512 (Accession # O75094) with two N-terminal Tags, His-tag and S-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 429836873
• NCBI GeneID: 6586
• NCBI Accession #: NP_001258875.1
• NCBI GenBank Nucleotide #: NM_001271946.1
• UniProt Accession #: O75094; O95804; Q9UFH5; A6H8U9
• Molecular Weight: 17.6kDa
• NCBI Official Full Name: slit homolog 3 protein isoform 1
• NCBI Official Synonym Full Names: slit homolog 3 (Drosophila)
• NCBI Official Symbol: SLIT3
• NCBI Official Synonym Symbols: MEGF5; SLIL2; SLIT1; slit2; Slit-3
• NCBI Protein Information: slit homolog 3 protein; multiple EGF-like domains protein 5; multiple epidermal growth factor-like domains protein 5
• UniProt Protein Name: Slit homolog 3 protein
• Protein Family: Slit homolog 3 protein
• UniProt Gene Name: SLIT3
• UniProt Synonym Gene Names: KIAA0814; MEGF5; SLIL2; Slit-3
• UniProt Entry Name: SLIT3_HUMAN

NCBI Description

The protein encoded by this gene is secreted, likely interacting with roundabout homolog receptors to effect cell migration. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]

Uniprot Description

SLIT3: May act as molecular guidance cue in cellular migration, and function may be mediated by interaction with roundabout homolog receptors. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: extracellular space; mitochondrion

Molecular Function: Roundabout binding; calcium ion binding

Biological Process: axon extension involved in axon guidance; negative regulation of cell proliferation; chemorepulsion involved in embryonic olfactory bulb interneuron migration; axon guidance; organ morphogenesis; response to cortisol stimulus; negative chemotaxis; negative regulation of cell growth; cellular response to hormone stimulus