Mouse CTDSP1 Monoclonal Antibody | anti-CTDSP1 antibody

CTDSP1 antibody

Cat. Num. AAA533191

• Gene Names: CTDSP1; NIF3; SCP1; NLIIF; NLI-IF
• Applications: Western Blot
• Purity: CTDSP1 antibody was purified by affinity chromatography.
• Synonyms: CTDSP1; Monoclonal Antibody; CTDSP1 antibody; Monoclonal CTDSP1; Anti-CTDSP1; Carboxy-terminal domain; RNA polymerase II protein; polypeptide A phosphatase 1; NLIIF; SCP1; NIF3; CTDSP 1; CTDSP-1; anti-CTDSP1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1A7
• Purity/Purification: CTDSP1 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 261

• Applicable Applications for anti-CTDSP1 antibody: Western Blot (WB)
• Application Notes: WB: 1:2000
• Biological Significance: Carboxy-terminal domain, RNA polymerase II, polypeptide A phosphatase 1, also known as CTDSP1, is a class 2C phosphatase with activity dependent on the conserved DxD motif. CTDSP1 preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Also it negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation.
• Cross-Reactivity: Human
• Immunogen: CTDSP1 antibody was raised in mouse using a full length recombinant protein of human CTDSP1 (NP_067021) produced in E Coli as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot

(Western Blot analysis using CTDSP1 antibodyWestern Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant CTDSP1 protein (Right) or empty vector (Left) detected with CTDSP1 antibody)

Related Product Information for anti-CTDSP1 antibody

Mouse monoclonal CTDSP1 antibody

Product Categories/Family for anti-CTDSP1 antibody

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 10864009
• NCBI GeneID: 58190
• NCBI Accession #: NP_067021
• NCBI GenBank Nucleotide #: NM_021198.2
• UniProt Accession #: Q9GZU7; Q7Z5Q3; Q7Z5Q4; C9IYG0
• Molecular Weight: 29,128 Da
• NCBI Official Full Name: carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 isoform 1
• NCBI Official Synonym Full Names: CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 1
• NCBI Official Symbol: CTDSP1
• NCBI Official Synonym Symbols: NIF3; SCP1; NLIIF; NLI-IF
• NCBI Protein Information: carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1
• UniProt Protein Name: Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1
• Protein Family: Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase
• UniProt Gene Name: CTDSP1
• UniProt Synonym Gene Names: NIF3; NLIIF; SCP1; NLI-IF; NLI-interacting factor 3; SCP1; Small CTD phosphatase 1
• UniProt Entry Name: CTDS1_HUMAN

NCBI Description

This gene encodes a member of the small C-terminal domain phosphatase (SCP) family of nuclear phosphatases. These proteins play a role in transcriptional regulation through specific dephosphorylation of phosphoserine 5 within tandem heptapeptide repeats of the C-terminal domain of RNA polymerase II. The encoded protein plays a role in neuronal gene silencing in non-neuronal cells, and may also inhibit osteoblast differentiation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

CTDSP1: Preferentially catalyzes the dephosphorylation of 'Ser- 5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: nucleus

Molecular Function: protein binding; metal ion binding; CTD phosphatase activity

Biological Process: regulation of transcription from RNA polymerase II promoter; negative regulation of neuron differentiation; negative regulation of protein amino acid phosphorylation; protein amino acid dephosphorylation