Human Solute carrier family 40 member 1 ELISA Kit | SLC40A1 elisa kit

Human Solute carrier family 40 member 1 ELISA Kit

Cat. Num. AAA761379

• Gene Names: SLC40A1; FPN1; HFE4; MTP1; IREG1; MST079; MSTP079; SLC11A3
• Reactivity: Human
• Synonyms: Solute carrier family 40 member 1; Human Solute carrier family 40 member 1 ELISA Kit; SLC40A1 (Solute carrier family 40 member 1)/FPN1/HFE4/MTP1/IREG1/MST079/MSTP079/SLC11A3/Ferroportin-1/Iron-regulated transporter 1; SLC40A1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SLC26A4. No significant cross-reactivity or interference between SLC26A4 and analogues was observed.
• Sequence Length: 571

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.78-50ng/ml
• Sensitivity: <0.469ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SLC26A4 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SLC26A4 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SLC40A1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-SLC26A4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-SLC26A4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the SLC26A4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of SLC26A4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 7657100
• NCBI GeneID: 30061
• NCBI Accession #: NP_055400.1
• NCBI GenBank Nucleotide #: NM_014585.5
• Molecular Weight: 62,542 Da
• NCBI Official Full Name: solute carrier family 40 member 1
• NCBI Official Synonym Full Names: solute carrier family 40 (iron-regulated transporter), member 1
• NCBI Official Symbol: SLC40A1
• NCBI Official Synonym Symbols: FPN1; HFE4; MTP1; IREG1; MST079; MSTP079; SLC11A3
• NCBI Protein Information: solute carrier family 40 member 1
• UniProt Protein Name: Solute carrier family 40 member 1
• Protein Family: Solute carrier family
• UniProt Gene Name: SLC40A1
• UniProt Synonym Gene Names: FPN1; IREG1; SLC11A3
• UniProt Entry Name: S40A1_HUMAN

NCBI Description

The protein encoded by this gene is a cell membrane protein that may be involved in iron export from duodenal epithelial cells. Defects in this gene are a cause of hemochromatosis type 4 (HFE4). [provided by RefSeq, Jul 2008]

Uniprot Description

SLC40A1: May be involved in iron export from duodenal epithelial cell and also in transfer of iron between maternal and fetal circulation. Mediates iron efflux in the presence of a ferroxidase (hephaestin and/or ceruloplasmin). Defects in SLC40A1 are the cause of hemochromatosis type 4 (HFE4). HFE4 is an autosomal dominant iron-loading disorder characterized by early iron accumulation in reticuloendothelial cells and a marked increase in serum ferritin before elevation of the transferrin saturation. Belongs to the ferroportin (FP) (TC 2.A.100) family. SLC40A subfamily.

Protein type: Transporter; Membrane protein, integral; Transporter, SLC family; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2q32

Cellular Component: basolateral plasma membrane; cytoplasm; integral to membrane; integral to plasma membrane; intracellular; multivesicular body; plasma membrane; synaptic vesicle

Molecular Function: ferrous iron transmembrane transporter activity; iron ion transmembrane transporter activity; peptide hormone binding; protein binding; receptor activity

Biological Process: cellular iron ion homeostasis; lymphocyte homeostasis; negative regulation of apoptosis; positive regulation of transcription from RNA polymerase II promoter; transmembrane transport

Disease: Hemochromatosis, Type 4