Human Zinc transporter 8 ELISA Kit | SLC30A8 elisa kit

Human Zinc transporter 8 ELISA Kit

Cat. Num. AAA761339

• Gene Names: Slc30a8; ZnT8; ZnT-8; C820002P14Rik
• Reactivity: Human
• Synonyms: Zinc transporter 8; Human Zinc transporter 8 ELISA Kit; ZNT8/SLC30A8/ZnT-8/Solute carrier family 30 member 8/zinc transporter 8/zinc transporter ZnT-8; SLC30A8 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SLC30A8. No significant cross-reactivity or interference between SLC30A8 and analogues was observed.
• Sequence Length: 367

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/ml
• Sensitivity: <0.188ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SLC30A8 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SLC30A8 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Standard Curve/Testing Data

Related Product Information for SLC30A8 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- SLC30A8 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- SLC30A8 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the SLC30A8 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of SLC30A8 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 27370218
• NCBI GeneID: 239436
• NCBI Accession #: NP_766404.1
• NCBI GenBank Nucleotide #: NM_172816.3
• Molecular Weight: 40,223 Da
• NCBI Official Full Name: zinc transporter 8
• NCBI Official Synonym Full Names: solute carrier family 30 (zinc transporter), member 8
• NCBI Official Symbol: Slc30a8
• NCBI Official Synonym Symbols: ZnT8; ZnT-8; C820002P14Rik
• NCBI Protein Information: zinc transporter 8
• UniProt Protein Name: Zinc transporter 8
• Protein Family: Zinc transporter
• UniProt Gene Name: Slc30a8
• UniProt Synonym Gene Names: Znt8; ZnT-8
• UniProt Entry Name: ZNT8_MOUSE

Uniprot Description

SLC30A8: Facilitates the accumulation of zinc from the cytoplasm into intracellular vesicles, being a zinc-efflux transporter. May be a major component for providing zinc to insulin maturation and/or storage processes in insulin-secreting pancreatic beta- cells. Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Transporter; Transporter, SLC family; Membrane protein, integral; Vesicle

Cellular Component: cytoplasmic membrane-bound vesicle; cytoplasmic vesicle; integral to membrane; membrane; plasma membrane; secretory granule

Molecular Function: cation transmembrane transporter activity; protein homodimerization activity; zinc ion transmembrane transporter activity

Biological Process: cation transport; cellular zinc ion homeostasis; glucose homeostasis; insulin secretion; ion transport; positive regulation of insulin secretion; response to glucose stimulus; response to zinc ion; sequestering of zinc ion; transmembrane transport; transport; zinc ion transport