Human solute carrier family 2 (facilitated glucose transporter) , member 4 ELISA Kit | SLC2A4 elisa kit

Human Glucose transporter 4, GLUT4 ELISA Kit

Cat. Num. AAA701745

• Gene Names: SLC2A4; GLUT4
• Reactivity: Human
• Synonyms: solute carrier family 2 (facilitated glucose transporter); member 4; Human Glucose transporter 4; GLUT4 ELISA Kit; GLUT4; glucose transporter 4; insulin-responsive glucose transporter type 4; SLC2A4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human SLC2A4. No significant cross-reactivity or interference between Human SLC2A4 and analogues was observed.
• Sequence Length: 509

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 62.5 pg/ml-4000 pg/ml
• Sensitivity: 15.6 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Transport
• Protein Biological Process 3: Sugar transport
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SLC2A4 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GLUT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GLUT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GLUT4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GLUT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4507011
• NCBI GeneID: 6517
• NCBI Accession #: NP_001033.1
• NCBI GenBank Nucleotide #: NM_001042.2
• UniProt Accession #: P14672; Q14CX2
• Molecular Weight: 54,787 Da
• NCBI Official Full Name: solute carrier family 2, facilitated glucose transporter member 4
• NCBI Official Synonym Full Names: solute carrier family 2 (facilitated glucose transporter), member 4
• NCBI Official Symbol: SLC2A4
• NCBI Official Synonym Symbols: GLUT4
• NCBI Protein Information: solute carrier family 2, facilitated glucose transporter member 4; GLUT-4; insulin-responsive glucose transporter type 4; glucose transporter type 4, insulin-responsive
• UniProt Protein Name: Solute carrier family 2, facilitated glucose transporter member 4
• Protein Family: Solute carrier family
• UniProt Gene Name: SLC2A4
• UniProt Synonym Gene Names: GLUT4; GLUT-4
• UniProt Entry Name: GTR4_HUMAN

NCBI Description

This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM). [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Insulin-regulated facilitative glucose transporter.