Rabbit CASP14 Antibody | anti-CASP14 antibody

Anti-CASP14 Antibody

Cat. Num. AAA7607419

• Gene Names: CASP14; ARCI12
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: CASP14; Anti-CASP14 Antibody; ARCI12; caspase-14; anti-CASP14 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Sequence Length: 242

• Applicable Applications for anti-CASP14 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: CASP14
• Preparation and Storage: Store at -20 degree C for 24 months. Avoid repeated freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescent analysis of MCF-7 cells using MBS7607419 (CASP14 antibody) at dilution of 1:100.)

Western Blot (WB)

(293T cells were subjected to SDS PAGE followed by western blot with MBS7607419 (CASP14 antibody) at dilution of 1:1000)

Related Product Information for anti-CASP14 antibody

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This caspase has been shown to be processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier.

NCBI and Uniprot Product Information

• NCBI GI #: 6912286
• NCBI GeneID: 23581
• NCBI Accession #: NP_036246.1
• NCBI GenBank Nucleotide #: NM_012114.3
• UniProt Accession #: P31944
• Molecular Weight: 30 kDa
• NCBI Official Full Name: caspase-14
• NCBI Official Synonym Full Names: caspase 14
• NCBI Official Symbol: CASP14
• NCBI Official Synonym Symbols: ARCI12
• NCBI Protein Information: caspase-14
• UniProt Protein Name: Caspase-14
• Protein Family: Caspase
• UniProt Gene Name: CASP14
• UniProt Synonym Gene Names: CASP-14
• UniProt Entry Name: CASPE_HUMAN

NCBI Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This caspase has been shown to be processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier. [provided by RefSeq, Jul 2008]

Uniprot Description

CASP14: Believed to be a non-apoptotic caspase which is involved in epidermal differentiation. Seems to play a role in keratinocyte differentiation and cornification. Probably regulates maturation of the epidermis by proteolytically processing filaggrin. Complex of unprocessed caspase-14 and processed 19 kDa (p19) and 10 kDa (p10) subunits. In undifferentiated keratinocytes under postconfluency growth conditions (in vitro). Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum). Expressed in keratinocytes of hair shaft and sebaceous glands. In psoriatic skin only expressed at very low levels. Belongs to the peptidase C14A family.

Protein type: Protease; EC 3.4.22.-

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: keratin filament; cytoplasm; nucleus

Molecular Function: cysteine-type endopeptidase activity

Biological Process: epidermis development; DNA damage response, signal transduction resulting in induction of apoptosis; keratinization; proteolysis