Human Calcium-binding mitochondrial carrier protein Aralar2 ELISA Kit | SLC25A13 elisa kit

Human Calcium-binding mitochondrial carrier protein Aralar2 ELISA Kit

Cat. Num. AAA764224

• Gene Names: SLC25A13; CTLN2; CITRIN; ARALAR2
• Reactivity: Human
• Synonyms: Calcium-binding mitochondrial carrier protein Aralar2; Human Calcium-binding mitochondrial carrier protein Aralar2 ELISA Kit; SLC25A13/ARALAR2/Citrin/Solute carrier family 25 member 13/Mitochondrial aspartate glutamate carrier 2; SLC25A13 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SLC25A13. No significant cross-reactivity or interference between SLC25A13 and analogues was observed.
• Sequence Length: 676

• Samples: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/ml
• Sensitivity: < 0.188ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SLC25A13were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SLC25A13were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SLC25A13 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- SLC25A13antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- SLC25A13 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the SLC25A13 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of SLC25A13 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 237649019
• NCBI GeneID: 10165
• NCBI Accession #: NP_001153682.1
• NCBI GenBank Nucleotide #: NM_001160210.1
• Molecular Weight: 74,304 Da
• NCBI Official Full Name: calcium-binding mitochondrial carrier protein Aralar2 isoform 1
• NCBI Official Synonym Full Names: solute carrier family 25 (aspartate/glutamate carrier), member 13
• NCBI Official Symbol: SLC25A13
• NCBI Official Synonym Symbols: CTLN2; CITRIN; ARALAR2
• NCBI Protein Information: calcium-binding mitochondrial carrier protein Aralar2
• UniProt Protein Name: Calcium-binding mitochondrial carrier protein Aralar2
• Protein Family: Calcium-binding mitochondrial carrier protein
• UniProt Gene Name: SLC25A13
• UniProt Synonym Gene Names: ARALAR2
• UniProt Entry Name: CMC2_HUMAN

NCBI Description

This gene is a member of the mitochondrial carrier family. The encoded protein contains four EF-hand Ca(2+) binding motifs in the N-terminal domain, and localizes to mitochondria. The protein catalyzes the exchange of aspartate for glutamate and a proton across the inner mitochondrial membrane, and is stimulated by calcium on the external side of the inner mitochondrial membrane. Mutations in this gene result in citrullinemia, type II. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

SLC25A13: Catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane. May have a function in the urea cycle. Defects in SLC25A13 are the cause of citrullinemia type 2 (CTLN2). Citrullinemia belongs to the urea cycle disorders. It is an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels. Ammonia intoxication is another manifestation. CTLN2 is characterized by neuropsychiatric symptoms including abnormal behaviors, loss of memory, seizures and coma. Death can result from brain edema. Onset is sudden and usually between the ages of 20 and 50 years. Defects in SLC25A13 are the cause of neonatal intrahepatic cholestasis due to citrin deficiency (NICCD). NICCD is a form of citrullinemia type 2 with neonatal onset. NICCD is characterized by suppression of the bile flow, hepatic fibrosis, low birth weight, growth retardation, hypoproteinemia, variable liver dysfunction. NICCD is generally not severe and symptoms disappear by one year of age with an appropriate diet. Years or even decades later, however, some individuals develop the characteristic features of citrullinemia type 2 with neuropsychiatric symptoms. Belongs to the mitochondrial carrier family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Membrane protein, integral; Membrane protein, multi-pass; Transporter, SLC family; Transporter

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: integral to plasma membrane; mitochondrial inner membrane; mitochondrion

Molecular Function: calcium ion binding; L-aspartate transmembrane transporter activity; L-glutamate transmembrane transporter activity; structural constituent of ribosome; transporter activity

Biological Process: aspartate transport; ATP biosynthetic process; carbohydrate metabolic process; cellular respiration; gluconeogenesis; glucose metabolic process; L-glutamate transport; malate-aspartate shuttle; response to calcium ion; translation; transport

Disease: Citrullinemia, Type Ii, Adult-onset; Citrullinemia, Type Ii, Neonatal-onset