Mouse Monocarboxylate transporter 2 ELISA Kit | Slc16a7 elisa kit

Mouse Monocarboxylate transporter 2 ELISA Kit

Cat. Num. AAA762634

• Gene Names: SLC16A7; MCT2
• Reactivity: Mouse
• Synonyms: Monocarboxylate transporter 2; Mouse Monocarboxylate transporter 2 ELISA Kit; Slc16a7/MCT 2/Solute carrier family 16 member 7; Slc16a7 elisa kit

• Reactivity: Mouse
• Sequence Length: 478

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Slc16a7 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Slc16a7 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Slc16a7 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Slc16a7 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Slc16a7 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 24431958
• NCBI GeneID: 9194
• NCBI Accession #: NP_004722.2
• NCBI GenBank Nucleotide #: NM_004731.4
• Molecular Weight: 52,200 Da
• NCBI Official Full Name: monocarboxylate transporter 2
• NCBI Official Synonym Full Names: solute carrier family 16 (monocarboxylate transporter), member 7
• NCBI Official Symbol: SLC16A7
• NCBI Official Synonym Symbols: MCT2
• NCBI Protein Information: monocarboxylate transporter 2
• UniProt Protein Name: Monocarboxylate transporter 2
• Protein Family: Monocarboxylate transporter
• UniProt Gene Name: SLC16A7
• UniProt Synonym Gene Names: MCT2; MCT 2
• UniProt Entry Name: MOT2_HUMAN

NCBI Description

This gene is a member of the monocarboxylate transporter family. Members in this family transport metabolites, such as lactate, pyruvate, and ketone bodies. The protein encoded by this gene catalyzes the proton-linked transport of monocarboxylates and has the highest affinity for pyruvate. This protein has been reported to be more highly expressed in prostate and colorectal cancer specimens when compared to control specimens. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]

Uniprot Description

SLC16A7: Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. MCT2 is a high affinity pyruvate transporter. Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.

Protein type: Transporter, SLC family; Membrane protein, integral; Membrane protein, multi-pass; Transporter

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: cytoplasm; integral to plasma membrane; plasma membrane

Molecular Function: lactate transmembrane transporter activity; pyruvate secondary active transmembrane transporter activity; pyruvate transmembrane transporter activity; symporter activity

Biological Process: transmembrane transport