Mouse RPTOR Monoclonal Antibody | anti-RPTOR antibody

RPTOR Antibody

Cat. Num. AAA9200487

• Gene Names: RPTOR; KOG1; Mip1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified Mouse Monoclonal Antibody (Mab)
• Synonyms: RPTOR; Monoclonal Antibody; RPTOR Antibody; Regulatory-associated protein of mTOR; Raptor; p150 target of rapamycin (TOR)-scaffold protein; KIAA1303; RAPTOR; anti-RPTOR antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1411CT316.2.151.34
• Specificity: This RPTOR antibody is generated from a mouse immunized with recombinant protein.
• Purity/Purification: Purified Mouse Monoclonal Antibody (Mab)
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Sequence Positions: 1005-1329

• Applicable Applications for anti-RPTOR antibody: ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC~~1:25; WB~~1:500-1:1000
• Function: Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response tonutrient and hormonal signals; functions as ascaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved inciliogenesis
• Cellular Location: Cytoplasm. Lysosome. Cytoplasmic granule. Note=Targeting to lysosomes depends onamino acid availability. In arsenite-stressed cells, accumulates in stress granules when associated with SPAG5 and association with lysosomes is drastically decreased
• Tissue Location: Highly expressed in skeletal muscle, and in alesser extent in brain, lung, small intestine,kidney and placenta Isoform 3 is widely expressed, with highest levels in nasalmucosa and pituitary and lowest in spleen
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using RPTOR Antibody. MBS9200487 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

Western Blot (WB)

(Western blot analysis of lysates from Hela, MCF-7, mouse C2C12, mouse NIH/3T3, rat C6 cell line (from left to right), using RPTOR Antibody. MBS9200487 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

Related Product Information for anti-RPTOR antibody

Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis.

Product Categories/Family for anti-RPTOR antibody

Crown Antibodies; Cell Biology; Signal Transduction

References

Kim D.-H.,et al.Cell 110:163-175(2002).
Hara K.,et al.Cell 110:177-189(2002).
Zody M.C.,et al.Nature 440:1045-1049(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Nagase T.,et al.DNA Res. 7:65-73(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 251823858
• NCBI GeneID: 57521
• NCBI Accession #: NP_001156506.1
• NCBI GenBank Nucleotide #: NM_001163034.1
• UniProt Accession #: Q8N122; Q8N4V9; Q8TB32; Q9P2P3; B2RN36; C6KEF2; F5H7J5
• Molecular Weight: 149038
• NCBI Official Full Name: regulatory-associated protein of mTOR isoform 2
• NCBI Official Synonym Full Names: regulatory associated protein of MTOR, complex 1
• NCBI Official Symbol: RPTOR
• NCBI Official Synonym Symbols: KOG1; Mip1
• NCBI Protein Information: regulatory-associated protein of mTOR
• UniProt Protein Name: Regulatory-associated protein of mTOR
• Protein Family: Regulatory-associated protein
• UniProt Gene Name: RPTOR
• UniProt Synonym Gene Names: KIAA1303; RAPTOR; Raptor
• UniProt Entry Name: RPTOR_HUMAN

NCBI Description

This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

Raptor: Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Interacts with MTOR. Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Binds directly to 4EBP1 and RPS6KB1 independently of its association with MTOR. Binds preferentially to poorly or non-phosphorylated forms of EIF4EBP1, and this binding is critical to the ability of MTOR to catalyze phosphorylation. Forms a complex with MTOR under both leucine-rich and -poor conditions. Interacts with ULK1 in a nutrient-dependent manner; the interaction is reduced during starvation. Interacts (when phosphorylated by AMPK) with 14-3-3 protein, leading to inhibit its activity. Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. Belongs to the WD repeat RAPTOR family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: nucleoplasm; cell soma; intracellular membrane-bound organelle; lysosome; lysosomal membrane; dendrite; cytoplasm; cytosol

Molecular Function: protein binding; protein complex binding; protein kinase binding

Biological Process: cellular response to nutrient levels; TOR signaling pathway; insulin receptor signaling pathway; positive regulation of transcription from RNA polymerase III promoter; positive regulation of endothelial cell proliferation; cell cycle arrest; cell growth; regulation of cell size; positive regulation of TOR signaling pathway