Rat Secondary Lymphoid Tissue Chemokine ELISA Kit | SLC elisa kit

Rat Secondary Lymphoid Tissue Chemokine ELISA Kit

Cat. Num. AAA726933

• Gene Names: CCL21; ECL; SLC; CKb9; TCA4; 6Ckine; SCYA21
• Reactivity: Rat
• Synonyms: Secondary Lymphoid Tissue Chemokine; Rat Secondary Lymphoid Tissue Chemokine ELISA Kit; SLC elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 100-2500pg/mL
• Sensitivity: 1.0pg/ml
• Intended Uses: This CCL-21 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CCL-21. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SLC elisa kit

Principle of the Assay: CCL-21 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCL-21 antibody and an CCL-21-HRP conjugate. The assay sample and buffer are incubated together with CCL-21-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCL-21 concentration since CCL-21 from samples and CCL-21-HRP conjugate compete for the anti-CCL-21 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCL-21 from the sample, fewer sites are left to bind CCL-21-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCL-21 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for SLC elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 4128129
• NCBI GeneID: 6366
• UniProt Accession #: O00585
• Molecular Weight: 14,646 Da
• NCBI Official Full Name: secondary lymphoid tissue chemokine
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 21
• NCBI Official Symbol: CCL21
• NCBI Official Synonym Symbols: ECL; SLC; CKb9; TCA4; 6Ckine; SCYA21
• NCBI Protein Information: C-C motif chemokine 21; beta chemokine exodus-2; secondary lymphoid tissue chemokine; Efficient Chemoattractant for Lymphocytes; small inducible cytokine subfamily A (Cys-Cys), member 21
• UniProt Protein Name: C-C motif chemokine 21
• UniProt Gene Name: CCL21
• UniProt Synonym Gene Names: SCYA21; UNQ784/PRO1600; SLC
• UniProt Entry Name: CCL21_HUMAN

NCBI Description

This antimicrobial gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19). [provided by RefSeq, Sep 2014]

Uniprot Description

CCL21: Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Chemokine; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine receptor binding; chemokine activity; CCR7 chemokine receptor binding

Biological Process: positive regulation of filopodium formation; positive regulation of I-kappaB kinase/NF-kappaB cascade; formation of immunological synapse; positive regulation of chemotaxis; positive regulation of dendritic cell antigen processing and presentation; cell maturation; positive regulation of JNK cascade; positive regulation of phosphoinositide 3-kinase activity; positive regulation of cell adhesion mediated by integrin; positive regulation of receptor-mediated endocytosis; G-protein coupled receptor protein signaling pathway; positive regulation of protein kinase B signaling cascade; positive regulation of pseudopodium formation; positive regulation of actin filament polymerization; cell-cell signaling; release of sequestered calcium ion into cytosol; positive regulation of protein kinase activity; T cell costimulation; ruffle organization and biogenesis; dendritic cell chemotaxis; establishment of T cell polarity; immune response; inflammatory response; positive regulation of cell-matrix adhesion