Signaling Lymphocytic Activation Molecule Family Recombinant Protein | SLAMF1 recombinant protein

Recombinant Signaling Lymphocytic Activation Molecule Family, Member 1 (SLAMF1)

Cat. Num. AAA2031398

• Gene Names: SLAMF1; SLAM; CD150; CDw150
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Signaling Lymphocytic Activation Molecule Family; Recombinant Signaling Lymphocytic Activation Molecule Family; Member 1 (SLAMF1); SLAMF1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LAVE LSYGTGTGMM DCPVILQELG RDTWLALTNE HQINKSANKS IRILVTMARS PGSKTNKKIV SFDLSKGGYP DHLEDGYQFQ SKNLSLKILG NRRESEGLYF VTVEENVSVH QSCTQLKLYE QVSTPEIKVL NKTQEENGTC HLLLACTVKR GDNVSYSWGD EAGTRLPSQA NRSHLLHLIL SNQHQDSIYNCTASNPVSSR SRTFNLW
• Sequence Length: 335

• Applicable Applications for SLAMF1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 27.4kDa
• Accurate Molecular Mass (KD): 27kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag, T7 tag
• Organism Species: Rattus norvegicus (Rat)
• Fragment: Leu17~Trp227
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4506969
• NCBI GeneID: 6504
• NCBI Accession #: NP_003028.1
• NCBI GenBank Nucleotide #: NM_003037.3
• Molecular Weight: 33,839 Da
• NCBI Official Full Name: signaling lymphocytic activation molecule
• NCBI Official Synonym Full Names: signaling lymphocytic activation molecule family member 1
• NCBI Official Symbol: SLAMF1
• NCBI Official Synonym Symbols: SLAM; CD150; CDw150
• NCBI Protein Information: signaling lymphocytic activation molecule
• UniProt Protein Name: Signaling lymphocytic activation molecule
• Protein Family: Signaling lymphocytic activation molecule
• UniProt Gene Name: SLAMF1
• UniProt Synonym Gene Names: SLAM
• UniProt Entry Name: SLAF1_HUMAN

Uniprot Description

SLAM: a type I membrane protein present on the surface of B cells, T cells and CD83+ dendritic cells. A high-affinity self-ligand considered to be important in bidirectional T <-> B-cell stimulation. SLAM-induced signal-transduction events in T lymphocytes are different from those in B cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A (SAP) acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (SHP-2)-dependent signal transduction operates. Its cytoplasmic domain interacts with SAP through part of its SH2 domain, and with SHP-2. Three alternatively spliced isoforms have been described.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: integral to membrane; phagocytic vesicle; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; antigen binding

Biological Process: viral reproduction; regulation of catalytic activity; regulation of vesicle fusion; positive regulation of cell proliferation; lymphocyte activation; signal transduction