Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Stromelysin-3 (MMP11) Standard Curve.)
Mouse Stromelysin-3 (MMP11) ELISA Kit | SL-3 elisa kit
Mouse Stromelysin-3 (MMP11) ELISA Kit
Gene Names
Mmp11; ST3; SL-3; Stmy3; MMP-11
Reactivity
Mouse
Synonyms
Stromelysin-3 (MMP11); Mouse Stromelysin-3 (MMP11) ELISA Kit; MMP11; ST3; STMY3; matrix metalloproteinase 11; matrix metalloproteinase 11 (stromelysin 3); stromelysin 3; stromelysinI; SL-3 elisa kit
Reactivity
Mouse
Specificity
Mouse Stromelysin-3 (MMP11) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MMP11. No significant cross-reactivity or interference between Mouse MMP11 and analogues was observed.
Sequence Length
492
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Stromelysin-3 (MMP11) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Stromelysin-3 (MMP11) Standard Curve.)
Related Product Information for SL-3 elisa kit
Description: This Mouse Stromelysin-3 (MMP11) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP11 in samples. An antibody specific for MMP11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP11 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Mmp11 encodes a member of the matrix metalloproteinase family of endopeptidases that are involved in remodeling extracellular matrix during, for example, embryonic development and tumor progression. The encoded protein undergoes post-translational proteolytic processing by furin endopeptidase to form an active enzyme. Subcutaneous introduction of cells expressing the encoded protein into nude mice results in increased tumor incidence. Mice lacking the encoded protein exhibit a decreased incidence of chemically-induced tumors. Alternative splicing results in multiple transcript variants.
Background: Mmp11 encodes a member of the matrix metalloproteinase family of endopeptidases that are involved in remodeling extracellular matrix during, for example, embryonic development and tumor progression. The encoded protein undergoes post-translational proteolytic processing by furin endopeptidase to form an active enzyme. Subcutaneous introduction of cells expressing the encoded protein into nude mice results in increased tumor incidence. Mice lacking the encoded protein exhibit a decreased incidence of chemically-induced tumors. Alternative splicing results in multiple transcript variants.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
stromelysin-3 preproprotein
NCBI Official Synonym Full Names
matrix metallopeptidase 11
NCBI Official Symbol
Mmp11
NCBI Official Synonym Symbols
ST3; SL-3; Stmy3; MMP-11
NCBI Protein Information
stromelysin-3
UniProt Protein Name
Stromelysin-3
UniProt Gene Name
Mmp11
UniProt Synonym Gene Names
SL-3; ST3; MMP-11
NCBI Description
This gene encodes a member of the matrix metalloproteinase family of endopeptidases that are involved in remodeling extracellular matrix during, for example, embryonic development and tumor progression. The encoded protein undergoes post-translational proteolytic processing by furin endopeptidase to form an active enzyme. Subcutaneous introduction of cells expressing the encoded protein into nude mice results in increased tumor incidence. Mice lacking the encoded protein exhibit a decreased incidence of chemically-induced tumors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
Uniprot Description
May play an important role in the progression of epithelial malignancies.
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Product Notes
The Mouse SL-3 mmp11 (Catalog #AAA9717620) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717620 ELISA Kit recognizes Mouse SL-3. It is sometimes possible for the material contained within the vial of "Stromelysin-3 (MMP11), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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